Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,284,931 A→G T83A (ACC→GCC)  agaC → N‑acetylgalactosamine‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,284,9310AG100.0% 31.3 / NA 12T83A (ACC→GCC) agaCN‑acetylgalactosamine‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/6);  total (6/6)

CTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCACCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCAAAGCC  >  NC_000913/3284799‑3285059
                                                                                                                                    |                                                                                                                                
cTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCa                                                                                                                            >  2:99544/1‑139 (MQ=255)
                    tGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGtt                                                                                                        <  1:99544/139‑1 (MQ=255)
                                     tAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAAt                                                                                       <  1:77504/139‑1 (MQ=255)
                                                                    cGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGtt                                                                     <  1:413690/126‑1 (MQ=255)
                                                                    cGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGtt                                                                     >  2:413690/1‑126 (MQ=255)
                                                                                 ccTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTc                                           <  2:55623/139‑1 (MQ=255)
                                                                                           gtgtTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGGCTTGGCCTGCCGTTTAGTTTGTTTATGCAGTACGTCATTCTGTTCTTCTa                                 >  2:216262/1‑139 (MQ=255)
                                                                                                   ccgccCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCACGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGGCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCtt                         >  2:158295/1‑139 (MQ=255)
                                                                                                                     gCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCa       <  2:4321/139‑1 (MQ=255)
                                                                                                                      cGGGTCTGATGACCGCCGTCATTGCATGGTCTTCGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTTTTAATGCAGTACGTCATTTTGTTTTTCTATTCCGCTTTCTCATTATTTTTGACCaa      >  1:4321/1‑139 (MQ=255)
                                                                                                                       gggTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCaaa     <  2:445757/139‑1 (MQ=255)
                                                                                                                          tCTGATGACCGCCCTCCTTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTTATGCAGTACGTCCTTCTGTTCTTCTATTCCGCTTTCTCCTTATTTTTTACCAAAgac  >  1:18183/1‑137 (MQ=255)
                                                                                                                                    |                                                                                                                                
CTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCACCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCAAAGCC  >  NC_000913/3284799‑3285059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: