Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,868,399	C→T	C304Y (TGT→TAT)	yidR ←	DUF3748 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,868,399	0	C	T	100.0%	78.8 / NA	23	C304Y (TGT→TAT)	yidR	DUF3748 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

AACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTG  >  NC_000913/3868300‑3868489                                                                                                    |                                                                                           aaCTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGAt                                                     <  2:1517/139‑1 (MQ=255)  aCTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAAcc                                                           <  1:284656/132‑1 (MQ=255)  aCTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAAcc                                                           >  2:284656/1‑132 (MQ=255)      gcgcGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACTCCGGAt                                                     <  2:392640/134‑1 (MQ=255)      gcgcGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACTCCGGAt                                                     >  1:392640/1‑134 (MQ=255)      gcgcGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGcc                                                <  2:193577/139‑1 (MQ=255)      gcgcGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGcc                                                <  1:56625/139‑1 (MQ=255)         cgGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACg                                                                     >  1:28199/1‑115 (MQ=255)         cgGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACg                                                                     <  2:28199/115‑1 (MQ=255)                 cgccCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGt                                     <  2:83569/139‑1 (MQ=255)                               aTAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTaaa                           >  2:213588/1‑135 (MQ=255)                               aTAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTaaa                           <  1:213588/135‑1 (MQ=255)                                        aGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCacga              >  1:47601/1‑139 (MQ=255)                                        aGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCacga              >  1:424999/1‑139 (MQ=255)                                                  tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGAc                                                                <  1:433987/79‑1 (MQ=255)                                                  tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGAc                                                                >  2:433987/1‑79 (MQ=255)                                                  tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCgg    >  1:47157/1‑139 (MQ=255)                                                  tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCgg    >  1:256650/1‑139 (MQ=255)                                                    ccGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTg  >  1:369684/1‑139 (MQ=255)                                                                                             cGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCa         <  1:308369/91‑1 (MQ=255)                                                                                             cGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCa         >  2:308369/1‑91 (MQ=255)                                                                                              ggATTATAGCGCGCCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCa                 <  2:258097/82‑1 (MQ=255)                                                                                              ggATTATAGCGCGCCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCa                 >  1:258097/1‑82 (MQ=255)                                                                                                    |                                                                                           AACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTG  >  NC_000913/3868300‑3868489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑