Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,936,381 C→T T35M (ACG→ATG)  rbsB → D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,936,3810CT100.0% 95.8 / NA 28T35M (ACG→ATG) rbsBD‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/17);  total (11/17)

ATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTGCAGGACTTAAC  >  NC_000913/3936253‑3936513
                                                                                                                                   |                                                                                                                                    
ataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTaacaac                                                                                                                            <  2:371918/139‑1 (MQ=255)
ataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTaacaac                                                                                                                            <  1:45053/139‑1 (MQ=255)
        tACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCtt                                                                                                                    >  2:312671/1‑139 (MQ=255)
           aGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGt                                                                                                                 <  1:261788/139‑1 (MQ=255)
            ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTa                                                                                                                <  2:114428/139‑1 (MQ=255)
             gACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTAt                                                                                                               <  1:98281/139‑1 (MQ=255)
               cATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTa                                                                                                                >  1:26090/1‑139 (MQ=255)
                 tCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGACAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCt                                                                                                           <  2:214818/139‑1 (MQ=255)
                  cTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTg                                                                                                          >  2:418601/1‑139 (MQ=255)
                             aCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgc                                                                                               >  1:449816/1‑139 (MQ=255)
                                           gCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAAccc                                                                                                                          >  1:243271/1‑98 (MQ=255)
                                           gCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAAccc                                                                                                                          <  2:243271/98‑1 (MQ=255)
                                              aCCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATa                                                                              <  1:418601/139‑1 (MQ=255)
                                                  tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                                                                          <  2:138764/139‑1 (MQ=255)
                                                  tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                                                                          <  1:29013/139‑1 (MQ=255)
                                                  tGGTTTCCGCTGTGCCGCTAAGCGCCACCGTCATTGCGAATGCGATGGCAAAAGACACCTTCGCGC‑‑‑CGGTGGACTCCATGCTTCACACCCCGTTCTTTGTAGCGCGGAAAGATTGTGAGCAGAAAGAGTAGGATAATCt                                                                          <  1:143702/139‑1 (MQ=255)
                                                        ccGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCta                                                                    <  1:317493/139‑1 (MQ=255)
                                                         cGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAAccc                                                                                                                          >  1:36069/1‑84 (MQ=255)
                                                         cGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAAccc                                                                                                                          <  2:36069/84‑1 (MQ=255)
                                                           cTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCta                                                                    <  2:26090/139‑1 (MQ=255)
                                                                      aGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGc                                                      <  2:449816/139‑1 (MQ=255)
                                                                       gcgcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCt                                                     <  2:144113/139‑1 (MQ=255)
                                                                                                           ccATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAc                                                                           >  1:475732/1‑81 (MQ=255)
                                                                                                           ccATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAc                                                                           <  2:475732/81‑1 (MQ=255)
                                                                                                            cATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcg                                                                                                >  1:445995/1‑59 (MQ=255)
                                                                                                            cATCGCGC‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcg                                                                                                <  2:445995/59‑1 (MQ=255)
                                                                                                                   c‑‑‑TGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTGCAgg         >  1:25352/1‑139 (MQ=255)
                                                                                                                       tggtggTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTGCAGGa        >  2:349241/1‑139 (MQ=255)
                                                                                                                             tctcCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTGCAGGACTTAAc  >  2:268815/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                    
ATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGC‑‑‑TGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTGCAGGACTTAAC  >  NC_000913/3936253‑3936513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: