Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	630,819	C→T	A309V (GCG→GTG)	cstA →	carbon starvation protein involved in peptide utilization, APC peptide transporter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	630,819	0	C	T	100.0%	92.3 / NA	27	A309V (GCG→GTG)	cstA	carbon starvation protein involved in peptide utilization, APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

GGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAT  >  NC_000913/630706‑630927                                                                                                                  |                                                                                                             ggTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACt                                                                                     >  2:217635/1‑139 (MQ=255)    gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGc                                                                                  <  2:56834/139‑1 (MQ=255)    gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGc                                                                                  <  2:395473/139‑1 (MQ=255)    gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGc                                                                                  >  1:332974/1‑139 (MQ=255)    gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGc                                                                                  <  2:332974/139‑1 (MQ=255)    gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGc                                                                                  <  1:67351/139‑1 (MQ=255)    gtgGTTACTGCAGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGc                                                                                  <  2:332387/139‑1 (MQ=255)                 gCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCg                                                                     >  2:418642/1‑139 (MQ=255)                        gTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCt                                                              >  2:266294/1‑139 (MQ=255)                            cTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTc                                                          >  2:451292/1‑139 (MQ=255)                                      aCCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGt                                                >  2:86941/1‑139 (MQ=255)                                         ttCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTa                                             >  1:459696/1‑139 (MQ=255)                                          tCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                            >  2:168024/1‑139 (MQ=255)                                             tGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCAc                                         >  1:359288/1‑139 (MQ=255)                                                 aaTCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATc                                     >  2:176311/1‑139 (MQ=255)                                                 aaTCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATc                                     >  1:36964/1‑139 (MQ=255)                                                     gggACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCt                                 >  1:28137/1‑139 (MQ=255)                                                                      ggCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGa                                                                                            >  2:183489/1‑63 (MQ=255)                                                                      ggCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGa                                                                                            <  1:183489/63‑1 (MQ=255)                                                                      ggCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                >  2:467758/1‑139 (MQ=255)                                                                                   ttGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGa   <  1:176311/139‑1 (MQ=255)                                                                                    tGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt  <  1:418642/139‑1 (MQ=255)                                                                                    tGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt  <  1:168024/139‑1 (MQ=255)                                                                                              cccGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCa                                          <  2:216596/89‑1 (MQ=255)                                                                                              cccGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCa                                          >  1:216596/1‑89 (MQ=255)                                                                                                             gCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg       >  1:143977/1‑109 (MQ=255)                                                                                                             gCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg       <  2:143977/109‑1 (MQ=255)                                                                                                                  |                                                                                                             GGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAT  >  NC_000913/630706‑630927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑