Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,086,260	G→A	A87V (GCC→GTC)	pgaD ←	biofilm PGA synthase PgaCD, regulatory subunit, c‑di‑GMP‑stimulated activity and dimerization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,086,260	0	G	A	100.0%	81.7 / NA	24	A87V (GCC→GTC)	pgaD	biofilm PGA synthase PgaCD, regulatory subunit, c‑di‑GMP‑stimulated activity and dimerization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/10);  total (14/10)

CCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCG  >  NC_000913/1086152‑1086395                                                                                                             |                                                                                                                                        cccTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCa                                                                                                           <  2:78289/139‑1 (MQ=255)                tgtACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAgcg                                                                                            >  2:450791/1‑139 (MQ=255)                 gtACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAgcgc                                                                                           >  2:453271/1‑139 (MQ=255)                 gtACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAgcgc                                                                                           >  1:54045/1‑139 (MQ=255)                              gtgGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCa                                                                                                           <  1:323861/110‑1 (MQ=255)                              gtgGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCa                                                                                                           >  2:323861/1‑110 (MQ=255)                                ggCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGaa                                                                                                                 <  2:9631/102‑1 (MQ=255)                                ggCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGaa                                                                                                                 >  1:9631/1‑102 (MQ=255)                                               gCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCa                                                                                        <  1:500588/112‑1 (MQ=255)                                               gCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCa                                                                                        >  2:500588/1‑112 (MQ=255)                                                         cTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCGGCTTATTGTACAGCGCCCAg                                                                                       <  1:301828/103‑1 (MQ=255)                                                         cTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAg                                                                                       >  2:301828/1‑103 (MQ=255)                                                                      gCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGaa                                      >  1:76907/1‑139 (MQ=255)                                                                           gCTCTCTGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCgg                                 >  2:452518/1‑139 (MQ=255)                                                                            ctctctGCATATTCTTGCGGCGTATATTGGTAGACTGCa                                                                                                                                    >  1:43408/1‑39 (MQ=255)                                                                            ctctctGCATATTCTTGCGGCGTATATTGGTAGACTGCa                                                                                                                                    <  2:43408/39‑1 (MQ=255)                                                                               tctGCATATTCTTGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTc                             >  1:399136/1‑139 (MQ=255)                                                                                         cTTGCGGCCTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATAAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCt                   <  2:305172/139‑1 (MQ=255)                                                                                           tGCGGCGTATATTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCttt                 >  1:193139/1‑139 (MQ=255)                                                                                                  tataTTGGTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGta          >  1:120931/1‑139 (MQ=255)                                                                                                        ggTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAAcc    >  2:508439/1‑139 (MQ=255)                                                                                                         gTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAAccc   <  2:76907/139‑1 (MQ=255)                                                                                                         gTAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAAccc   <  1:450791/139‑1 (MQ=255)                                                                                                          tAGACTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCg  <  2:54045/139‑1 (MQ=255)                                                                                                             |                                                                                                                                        CCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCG  >  NC_000913/1086152‑1086395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑