| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,756,216 | (A)5→4 | coding (519/1413 nt) | pykF → | pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,756,212 | 0 | A | . | 100.0% | 156.6 / NA | 38 | coding (515/1413 nt) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (21/17); total (21/17) | |||||||||||
GTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACA > NC_000913/1756080‑1756346 | gTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGaaaagac > 1:53233/1‑136 (MQ=255) gATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGCCCTGGGCGAAAACAAAGGTGTGACCCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAAc < 1:109556/139‑1 (MQ=255) aaGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc < 2:445309/134‑1 (MQ=255) aaGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc > 1:445309/1‑134 (MQ=255) aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTgg > 1:16312/1‑139 (MQ=255) aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTgg > 1:398585/1‑139 (MQ=255) tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc < 1:827445/139‑1 (MQ=255) aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc < 2:527912/114‑1 (MQ=255) aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc > 1:527912/1‑114 (MQ=255) cAAAGTTAACTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAg > 1:731810/1‑139 (MQ=255) tGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgc < 2:431548/139‑1 (MQ=255) tAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctg < 2:394020/139‑1 (MQ=255) tAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctg < 1:968672/139‑1 (MQ=255) aaGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTgctgct > 2:992655/1‑139 (MQ=255) aGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc < 1:1017991/97‑1 (MQ=255) aGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc > 2:1017991/1‑97 (MQ=255) aacaacGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTAt > 1:567327/1‑139 (MQ=255) cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAg > 1:632009/1‑81 (MQ=255) cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAg < 2:632009/81‑1 (MQ=255) cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc < 2:895584/103‑1 (MQ=255) cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc > 1:895584/1‑103 (MQ=255) aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt > 2:300333/1‑133 (MQ=255) aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt < 1:300333/133‑1 (MQ=255) aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGt > 2:458812/1‑139 (MQ=255) ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACa < 2:315490/94‑1 (MQ=255) ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACa > 1:315490/1‑94 (MQ=255) ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGa > 1:609678/1‑139 (MQ=255) cGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACg < 2:936905/139‑1 (MQ=255) aaCAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTAt < 2:53233/139‑1 (MQ=255) cctgGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCt > 2:1804/1‑139 (MQ=255) tCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCa > 2:736888/1‑139 (MQ=255) tGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctgc < 2:766114/77‑1 (MQ=255) tGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctgc > 1:766114/1‑77 (MQ=255) tGCCAGCACTGGCTG‑AAATGACCAAGAGGACCTGATGTTTGGTTTTGAACAAGGCGTAGATTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGGTATAGAAATCCGTGAGCACCTGAAAGAGCATGGCGGCGaa > 2:957128/1‑139 (MQ=255) ccAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTaa > 2:459223/1‑85 (MQ=255) ccAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTaa < 1:459223/85‑1 (MQ=255) ccAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaaaa < 2:609678/139‑1 (MQ=255) ggCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCcaca > 2:147113/1‑139 (MQ=255) ggCTG‑AAAAGACAAACAGAACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCcaca > 2:625000/1‑139 (MQ=255) | GTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACA > NC_000913/1756080‑1756346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |