Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	199,121	C→T	V398V (GTC→GTT)	bamA →	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	199,121	0	C	T	100.0%	132.6 / NA	38	V398V (GTC→GTT)	bamA	BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/22);  total (16/22)

TTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCA  >  NC_000913/198986‑199249                                                                                                                                        |                                                                                                                                 ttttGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgat                                                                                                                               >  1:83023/1‑139 (MQ=255)    tGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTGGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAAGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgata                                                                                                                              <  1:686054/137‑1 (MQ=255)    tGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgata                                                                                                                              >  2:686054/1‑137 (MQ=255)     gAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgataccg                                                                                                                           <  1:870751/139‑1 (MQ=255)      aaGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgataccga                                                                                                                          >  1:964956/1‑139 (MQ=255)           aaCGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATAccc                                                                                                                     >  2:356351/1‑139 (MQ=255)              gATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAAc                                                                                                                  <  1:880917/139‑1 (MQ=255)              gATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAAc                                                                                                                  <  1:356351/139‑1 (MQ=255)              gATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAAc                                                                                                                  <  2:373462/139‑1 (MQ=255)                tACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACgt                                                                                                                >  2:575593/1‑139 (MQ=255)                   cTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgataccgatac                                                                                                                       <  2:918134/129‑1 (MQ=255)                   cTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgataccgatac                                                                                                                       >  1:918134/1‑129 (MQ=255)                   cTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGtt                                                                                                             <  1:575593/139‑1 (MQ=255)                       aaaGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCgg                                                                                                         <  1:227062/139‑1 (MQ=255)                             gCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGcc                                                                                                   >  2:552434/1‑139 (MQ=255)                                  ccTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGAc                                                                                              <  1:745532/139‑1 (MQ=255)                                     gCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAg                                                                                           >  1:903188/1‑139 (MQ=255)                                         cgcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg                                                                                       <  2:32971/139‑1 (MQ=255)                                         cgcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg                                                                                       <  2:903188/139‑1 (MQ=255)                                         cgcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTg                                                                                       <  1:1114755/139‑1 (MQ=255)                                           cgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGAt                                                                                     >  1:883596/1‑139 (MQ=255)                                            gAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATg                                                                                    <  1:663111/139‑1 (MQ=255)                                                tGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGTGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATgtcgt                                                                                >  2:644080/1‑139 (MQ=255)                                                   gTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTa                                                                             <  1:644080/139‑1 (MQ=255)                                                           gAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTaa                                                                     <  2:851086/139‑1 (MQ=255)                                                             aGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAAc                                                                                                                  >  1:212056/1‑92 (MQ=255)                                                             aGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAAc                                                                                                                  <  2:212056/92‑1 (MQ=255)                                                                          gggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgataccgatac                                                                                                                       <  2:181628/74‑1 (MQ=255)                                                                          gggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTtgataccgatac                                                                                                                       >  1:181628/1‑74 (MQ=255)                                                                                 aTCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCtt                                               >  2:997529/1‑139 (MQ=255)                                                                                  tCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTc                                              <  1:552434/139‑1 (MQ=255)                                                                                      gTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACt                                          >  1:301852/1‑139 (MQ=255)                                                                                       tCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACtt                                         <  1:997529/139‑1 (MQ=255)                                                                                         gATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTg                                       >  1:1012999/1‑139 (MQ=255)                                                                                            cAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTa                                    >  2:980450/1‑139 (MQ=255)                                                                                                 tAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGt                               <  2:624491/139‑1 (MQ=255)                                                                                                     gAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACg                           <  2:964956/139‑1 (MQ=255)                                                                                                         gTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTa                                                                             >  1:846094/1‑85 (MQ=255)                                                                                                         gTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTa                                                                             <  2:846094/85‑1 (MQ=255)                                                                                                         gTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcg                                                              <  2:709471/100‑1 (MQ=255)                                                                                                         gTCTGAATCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAgcg                                                              >  1:709471/1‑100 (MQ=255)                                                                                                               aTCGTCTGGGCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAg                 >  1:416028/1‑139 (MQ=255)                                                                                                                      gggCTTCTTTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTg          <  1:142330/139‑1 (MQ=255)                                                                                                                            cttTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGcc                                                                                                   >  1:953281/1‑44 (MQ=255)                                                                                                                            cttTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGcc                                                                                                   <  2:953281/44‑1 (MQ=255)                                                                                                                            cttTGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTc    >  1:841875/1‑139 (MQ=255)                                                                                                                              ttGAAACTGTTGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCa  <  2:617244/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 TTTTGAAGGTAACGATACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCA  >  NC_000913/198986‑199249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑