Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,339,927	A→G	S200G (AGC→GGC)	pgpB →	phosphatidylglycerophosphatase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,339,927	0	A	G	100.0%	178.0 / NA	47	S200G (AGC→GGC)	pgpB	phosphatidylglycerophosphatase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/27);  total (20/27)

ACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCATTAACACCACCTGCGGAAG  >  NC_000913/1339792‑1340056                                                                                                                                        |                                                                                                                                  aCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGgcc                                                                                                                                >  1:452018/1‑139 (MQ=255) aCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGgcc                                                                                                                                <  2:268565/139‑1 (MQ=255) aCGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGgcc                                                                                                                                >  1:914743/1‑139 (MQ=255) aCGGGGTTTGCATTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGgcc                                                                                                                                >  1:1076051/1‑139 (MQ=255)      gTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCctg                                                                                                                           <  2:209780/139‑1 (MQ=255)        ttGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCctgct                                                                                                                         <  2:912675/139‑1 (MQ=255)         tGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCctgctg                                                                                                                        <  1:799004/139‑1 (MQ=255)           ccTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTgctgct                                                                                                                      <  2:339568/139‑1 (MQ=255)               tccttccGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACGATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGTGAGGCCGCCTGCTGCTCggg                                                                                                                  <  2:614786/139‑1 (MQ=255)               tccttccGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCtgctgc                                                                                                                       <  2:434690/134‑1 (MQ=255)               tccttccGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCtgctgc                                                                                                                       >  1:434690/1‑134 (MQ=255)               tccttccGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCggg                                                                                                                  <  2:430876/139‑1 (MQ=255)               tccttccGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCggg                                                                                                                  <  2:915717/139‑1 (MQ=255)                             cGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCAcg                                                                                                    <  2:452018/139‑1 (MQ=255)                             cGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCAcg                                                                                                    <  2:1019516/139‑1 (MQ=255)                                tGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGa                                                                                                 >  2:520638/1‑139 (MQ=255)                                 gTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGAt                                                                                                <  1:407959/139‑1 (MQ=255)                                 gTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGAt                                                                                                <  2:197485/139‑1 (MQ=255)                                  tttGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATc                                                                                               <  2:438973/139‑1 (MQ=255)                                      ctgcCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGgt                                                                                           <  2:345112/139‑1 (MQ=255)                                                       ctgGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGtagtag                                                                                      <  1:954064/127‑1 (MQ=255)                                                       ctgGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGtagtag                                                                                      >  2:954064/1‑127 (MQ=255)                                                       ctgGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGAttt                                                                          >  1:184658/1‑139 (MQ=255)                                                          gCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGAt                                                                                                >  2:134829/1‑114 (MQ=255)                                                          gCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGAt                                                                                                <  1:134829/114‑1 (MQ=255)                                                             gTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTggg                                                                    <  2:409607/139‑1 (MQ=255)                                                               tGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGgcg                                                                  >  1:507508/1‑139 (MQ=255)                                                               tGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGgcg                                                                  >  1:455261/1‑139 (MQ=255)                                                                 gTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCt                                                                >  2:1043377/1‑139 (MQ=255)                                                                          ggCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTggtggcggt                                                       <  1:520638/139‑1 (MQ=255)                                                                                 tCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACg                                                >  2:643566/1‑139 (MQ=255)                                                                                        aCGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTg                                         <  2:1076051/139‑1 (MQ=255)                                                                                                      tATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTtgt                           <  2:475239/139‑1 (MQ=255)                                                                                                      tATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTtgt                           <  2:507508/139‑1 (MQ=255)                                                                                                       aTCTTGCTGGTCTGGGCAACGGGAGTCATGGGAGGCCGTCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTtgtg                          <  1:438171/139‑1 (MQ=255)                                                                                                         ctggCTGTTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGCGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTggg                        <  2:470112/136‑1 (MQ=255)                                                                                                             cTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCAt                    >  1:966167/1‑139 (MQ=255)                                                                                                             cTGGTCTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCAt                    >  2:477374/1‑139 (MQ=255)                                                                                                                  cTGGGCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCATTAaca               >  1:1099623/1‑139 (MQ=255)                                                                                                                     ggCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCATTAac                <  2:375803/135‑1 (MQ=255)                                                                                                                     ggCAACGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCATTAac                >  1:375803/1‑135 (MQ=255)                                                                                                                          cGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAAc                                                 <  2:480511/97‑1 (MQ=255)                                                                                                                          cGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAAc                                                 >  1:480511/1‑97 (MQ=255)                                                                                                                          cGGGAGTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCATTAACACCACCTGc       >  2:975893/1‑139 (MQ=255)                                                                                                                               gTCATGGGAGGCCGCCTGCTGCTCGGGTTGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCATTAACACCACCTGCGgaag  >  2:985066/1‑139 (MQ=255)                                                                                                                               gTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAAc                                                 >  2:627573/1‑92 (MQ=255)                                                                                                                               gTCATGGGAGGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAAc                                                 <  1:627573/92‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  ACGGGGTTTGCCTTTCCTTCCGGTCACACGATGTTTGCTGCCAGTTGGGCACTGCTGGCCGTTGGTTTGCTGTGGCCGCGTCGGCGAACGTTAACCATTGCTATCTTGCTGGTCTGGGCAACGGGAGTCATGGGAAGCCGCCTGCTGCTCGGGATGCATTGGCCACGCGATCTGGTAGTAGCTACGTTGATTTCGTGGGCGCTGGTGGCGGTGGCAACGTGGCTTGCGCAACGAATTTGTGGGCCATTAACACCACCTGCGGAAG  >  NC_000913/1339792‑1340056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑