Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,350,817	A→G	F75L (TTC→CTC)	fabI ←	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,350,817	0	A	G	100.0%	216.9 / NA	57	F75L (TTC→CTC)	fabI	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/27);  total (30/27)

GATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGC  >  NC_000913/1350683‑1350951                                                                                                                                       |                                                                                                                                       gATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATg                                                                                                                                    >  1:243693/1‑139 (MQ=255)   tGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGgt                                                                                                                                  >  1:87018/1‑139 (MQ=255)     tCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGgtgt                                                                                                                                <  1:1113946/139‑1 (MQ=255)            cAATTTTGAAGCCTTCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCt                                                                                                                         <  1:56380/139‑1 (MQ=255)                          tCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCaa                                                                                                           <  2:335412/139‑1 (MQ=255)                               ggTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCg                                                                                                      <  2:965053/139‑1 (MQ=255)                                 tAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa                                                                                                    <  2:1068542/139‑1 (MQ=255)                                 tAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCa                                                                                                    <  2:87018/139‑1 (MQ=255)                                    cGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTg                                                                                                 >  2:48461/1‑139 (MQ=255)                                               tAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGt                                                                                      <  2:53000/139‑1 (MQ=255)                                                  tCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCg                                                                                                      <  1:636134/120‑1 (MQ=255)                                                  tCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCg                                                                                                      >  2:636134/1‑120 (MQ=255)                                                    aCCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGc                                                                                                                      <  2:330368/102‑1 (MQ=255)                                                    aCCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGc                                                                                                                      >  1:330368/1‑102 (MQ=255)                                                      cATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAAcc                                                                               >  1:2178/1‑139 (MQ=255)                                                       aTCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAAccc                                                                              >  2:739611/1‑139 (MQ=255)                                                        tCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCaa                                                                                                           >  2:1107300/1‑109 (MQ=255)                                                        tCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCaa                                                                                                           <  1:1107300/109‑1 (MQ=255)                                                        tCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCa                                                                             >  1:140507/1‑139 (MQ=255)                                                          cAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAAt                                                                           >  2:590135/1‑139 (MQ=255)                                                            gCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCAtc                                                                                                                   <  1:967653/97‑1 (MQ=255)                                                            gCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCAtc                                                                                                                   >  2:967653/1‑97 (MQ=255)                                                                                 cAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTAcgcg                                                    <  1:739611/139‑1 (MQ=255)                                                                                  aaTAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTa                                                        <  1:287348/134‑1 (MQ=255)                                                                                  aaTAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTa                                                        >  2:287348/1‑134 (MQ=255)                                                                                   aTAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGc                                                  >  2:1092563/1‑139 (MQ=255)                                                                                     agagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCg                                                                                                      >  1:306729/1‑85 (MQ=255)                                                                                     agagTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCg                                                                                                      <  2:306729/85‑1 (MQ=255)                                                                                       agTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCttt                                              <  1:25811/139‑1 (MQ=255)                                                                                         tgtACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCa                                            >  1:849194/1‑139 (MQ=255)                                                                                          gtACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACAt                                                                                                        >  2:601581/1‑78 (MQ=255)                                                                                          gtACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACAt                                                                                                        <  1:601581/78‑1 (MQ=255)                                                                                          gtACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTAcg                                                      >  2:137835/1‑128 (MQ=255)                                                                                          gtACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTAcg                                                      <  1:137835/128‑1 (MQ=255)                                                                                          gtACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAg                                           >  2:704881/1‑139 (MQ=255)                                                                                          gtACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAg                                           >  2:652028/1‑139 (MQ=255)                                                                                          gtACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAg                                           >  2:609277/1‑139 (MQ=255)                                                                                           tACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTg                                                                                                              >  2:123944/1‑71 (MQ=255)                                                                                           tACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTg                                                                                                              <  1:123944/71‑1 (MQ=255)                                                                                           tACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGt                                          >  2:519221/1‑139 (MQ=255)                                                                                             cGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGttt                                        >  2:632766/1‑139 (MQ=255)                                                                                              gAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTg                                       <  1:1092563/139‑1 (MQ=255)                                                                                                  ccGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGt                                   >  1:694214/1‑139 (MQ=255)                                                                                                     tCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCa                                                                                                                     >  2:179854/1‑54 (MQ=255)                                                                                                     tCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCa                                                                                                                     <  1:179854/54‑1 (MQ=255)                                                                                                            tCGGCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGt                         >  2:297581/1‑139 (MQ=255)                                                                                                              ggCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTAcg                                                      >  1:705205/1‑108 (MQ=255)                                                                                                              ggCCAAACTTTCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTAcg                                                      <  2:705205/108‑1 (MQ=255)                                                                                                                       ttCCCCAGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGtt              <  1:930575/139‑1 (MQ=255)                                                                                                                             aGTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTc        <  2:2178/139‑1 (MQ=255)                                                                                                                              gTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGtt                                  <  1:153953/112‑1 (MQ=255)                                                                                                                              gTTCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGtt                                  >  2:153953/1‑112 (MQ=255)                                                                                                                               ttCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCt      <  2:140507/139‑1 (MQ=255)                                                                                                                                tCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCttt                                              >  2:985415/1‑98 (MQ=255)                                                                                                                                tCAGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCttt                                              <  1:985415/98‑1 (MQ=255)                                                                                                                                  aGCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTcg   >  1:1095435/1‑139 (MQ=255)                                                                                                                                   gCGAGCATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTcgc  <  1:864144/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GATGTCGTGGGCAATTTTGAAGCCTTCACGGGTAACGGCGTTAACATAGTCACCATCCAGCTGATCGCCAGGTGCAAAACCAATAGAGTGTACGAAACCGTCAAATTTCGGCCAAACTTTCCCCAGTTCAGCGAACATGGTGTCGATGCTGGCATCTTCTGCAACATCGCACTGCAGAACGATGTCAGAACCCAATTGAGCGGCAAATTCTTCTACGCGGCCTTTCAGTTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGC  >  NC_000913/1350683‑1350951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑