Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,430	G→A	P69L (CCG→CTG)	mepM ←	murein DD‑endopeptidase, space‑maker hydrolase, septation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,430	0	G	A	100.0%	256.1 / NA	72	P69L (CCG→CTG)	mepM	murein DD‑endopeptidase, space‑maker hydrolase, septation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/35);  total (37/35)

TACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGAC  >  NC_000913/1941298‑1941564                                                                                                                                     |                                                                                                                                       tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  1:50987/1‑139 (MQ=255) tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  2:846028/1‑139 (MQ=255) tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  1:572149/1‑139 (MQ=255) tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  2:728290/1‑139 (MQ=255) tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  1:918062/1‑139 (MQ=255) tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATAAGCTCAc                                                                                                                                  >  1:925704/1‑139 (MQ=255)  aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 >  2:308713/1‑139 (MQ=255)  aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:136620/139‑1 (MQ=255)  aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:423339/139‑1 (MQ=255)  aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:935400/139‑1 (MQ=255)  aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTCCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:231954/139‑1 (MQ=255)   cTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTg                                                                                                                                >  1:557704/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             <  2:370699/139‑1 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:1033446/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:251631/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:452363/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:572589/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:618423/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:719386/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:730868/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:370699/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:1003006/1‑139 (MQ=255)      aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             <  1:118893/139‑1 (MQ=255)       ttGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCtt                                                                                                                            <  1:455104/139‑1 (MQ=255)                    ttAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGcga                                                                                                               >  1:418795/1‑139 (MQ=255)                          tATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAAt                                                                                                         >  2:402546/1‑139 (MQ=255)                           aTCGCCAGTGGCAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAAtt                                                                                                        <  1:728290/139‑1 (MQ=255)                             cGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  1:471727/112‑1 (MQ=255)                             cGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 >  2:471727/1‑112 (MQ=255)                                cAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGtt                                                                                                   <  1:693304/139‑1 (MQ=255)                                  gTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCt                                                                                                 >  2:18608/1‑139 (MQ=255)                                    ggAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGt                                                                                               <  2:1033446/139‑1 (MQ=255)                                         caacaTATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAg                                                                                          <  2:276845/139‑1 (MQ=255)                                               atTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAAt                                                                                    <  1:377907/139‑1 (MQ=255)                                                     gCACGCCCGCTCCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGtttt                                                                              <  1:730868/139‑1 (MQ=255)                                                          cccGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:149548/83‑1 (MQ=255)                                                          cccGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 >  1:149548/1‑83 (MQ=255)                                                            cGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAAtt                                                                       <  1:618423/139‑1 (MQ=255)                                                              cTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTgg                                                                     >  1:565051/1‑139 (MQ=255)                                                              cTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTgg                                                                     <  2:50987/139‑1 (MQ=255)                                                                tCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATAAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCg                                                                   <  2:925704/139‑1 (MQ=255)                                                                 cACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGt                                                                  <  2:918062/139‑1 (MQ=255)                                                                         aTTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCAc                                                          <  2:1106882/139‑1 (MQ=255)                                                                                         cGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAgg                                          >  1:249087/1‑139 (MQ=255)                                                                                           tCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             <  2:126231/54‑1 (MQ=255)                                                                                           tCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:126231/1‑54 (MQ=255)                                                                                            ccTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTgg                                                                                                                               >  1:894738/1‑51 (MQ=255)                                                                                            ccTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTgg                                                                                                                               <  2:894738/51‑1 (MQ=255)                                                                                                gggAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAg                                   >  1:1123466/1‑139 (MQ=255)                                                                                                     tGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGc                              >  1:343335/1‑139 (MQ=255)                                                                                                     tGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGc                              <  1:308713/139‑1 (MQ=255)                                                                                                     tGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGc                              >  1:710291/1‑139 (MQ=255)                                                                                                      ggCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCg                             <  1:822797/139‑1 (MQ=255)                                                                                                       gCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGa                            <  2:719386/139‑1 (MQ=255)                                                                                                        cTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGAc                           >  2:938406/1‑139 (MQ=255)                                                                                                           cATCTTCTTGTGCAGCTTTATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGc                        <  1:846028/139‑1 (MQ=255)                                                                                                           cATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGc                        <  2:418795/139‑1 (MQ=255)                                                                                                           cATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCATGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGc                        <  1:643357/139‑1 (MQ=255)                                                                                                                       cAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATa                                             <  1:10722/106‑1 (MQ=255)                                                                                                                       cAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATa                                             >  2:10722/1‑106 (MQ=255)                                                                                                                       cAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAAc            >  1:74874/1‑139 (MQ=255)                                                                                                                          cTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCa                                                                                      >  2:365990/1‑62 (MQ=255)                                                                                                                          cTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCa                                                                                      <  1:365990/62‑1 (MQ=255)                                                                                                                          cTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGt         >  2:560119/1‑139 (MQ=255)                                                                                                                          cTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGt         >  2:412132/1‑139 (MQ=255)                                                                                                                           ttGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCa                                                           >  2:566208/1‑88 (MQ=255)                                                                                                                           ttGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCa                                                           <  1:566208/88‑1 (MQ=255)                                                                                                                              atcaatcaGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGc     <  1:251631/139‑1 (MQ=255)                                                                                                                               tCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCaa                                                                                     >  2:638509/1‑58 (MQ=255)                                                                                                                               tCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCaa                                                                                     <  1:638509/58‑1 (MQ=255)                                                                                                                                cAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGa   <  1:402546/139‑1 (MQ=255)                                                                                                                                 aaTCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGAc  <  1:18608/139‑1 (MQ=255)                                                                                                                                 aaTCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGACCAGAGTAAGAACGGTGAGCGAc  <  2:572149/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGAC  >  NC_000913/1941298‑1941564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑