Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,036,909 T→C Q415R (CAG→CGG)  yedV ← putative sensory kinase in two‑component regulatory system with YedW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,036,9090TC97.9% 344.3 / ‑2.4 93Q415R (CAG→CGG) yedVputative sensory kinase in two‑component regulatory system with YedW
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (49/42);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

TACTGAAGCCCTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATG  >  NC_000913/2036779‑2037042
                                                                                                                                  |                                                                                                                                     
tACTGAAGCCCTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACg                                                                                                                               >  1:149406/1‑139 (MQ=255)
tACTGAAGCCCTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACg                                                                                                                               >  1:964533/1‑139 (MQ=255)
 aCTGAAGCCCTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACgg                                                                                                                              >  1:806824/1‑139 (MQ=255)
         ccTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGAcgc                                                                                                                      >  1:366628/1‑139 (MQ=255)
          cTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGAcgcg                                                                                                                     >  2:814025/1‑139 (MQ=255)
          cTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGAcgcg                                                                                                                     >  2:288056/1‑139 (MQ=255)
            aTGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGaa                                                                                                                   >  2:214904/1‑139 (MQ=255)
            aTGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGaa                                                                                                                   >  1:869956/1‑139 (MQ=255)
                  aTTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTAtct                                                                                                             >  2:1067591/1‑139 (MQ=255)
                       tttGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCg                                                                                                        <  1:1122079/139‑1 (MQ=255)
                       tttGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCg                                                                                                        >  2:197593/1‑139 (MQ=255)
                         tGCGGTAACGTAATGCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGTCAATCGCTTTGACTAAAGAAAGGCCTCGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGcc                                                                                                      >  1:682593/1‑139 (MQ=255)
                         tGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGcc                                                                                                      >  1:558283/1‑139 (MQ=255)
                            ggTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCaaa                                                                                                   <  2:806824/139‑1 (MQ=255)
                              tAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAAt                                                                                                 <  1:947281/139‑1 (MQ=255)
                                     aTCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGa                                                                                          >  2:783746/1‑139 (MQ=255)
                                        cGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAaga                                                                                       <  2:869956/139‑1 (MQ=255)
                                           aaCACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGtt                                                                                    >  2:731065/1‑139 (MQ=255)
                                           aaCACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGtt                                                                                    >  1:248083/1‑139 (MQ=255)
                                                aTTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCa                                                                               >  2:338919/1‑139 (MQ=255)
                                                  tATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCGAAATCTACGGAAGAGTTTTTCAgg                                                                             <  1:686527/139‑1 (MQ=255)
                                                  tATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAgg                                                                             <  1:353038/139‑1 (MQ=255)
                                                   aTGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGc                                                                            <  1:814025/139‑1 (MQ=255)
                                                          ttGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTaa                                                                     >  2:459343/1‑139 (MQ=255)
                                                                 aGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGcc                                                                                                      <  1:900884/99‑1 (MQ=255)
                                                                 aGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGcc                                                                                                      >  2:900884/1‑99 (MQ=255)
                                                                  gTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGtt                                                             >  1:803890/1‑139 (MQ=255)
                                                                   tGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTc                                                            >  1:862393/1‑139 (MQ=255)
                                                                   tGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTc                                                            <  2:580692/139‑1 (MQ=255)
                                                                     aTACGTAGCACTTCCCCCATGCAATTCGGCAATCTCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCa                                                          >  1:585524/1‑139 (MQ=255)
                                                                      tACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAg                                                         >  1:966211/1‑139 (MQ=255)
                                                                       aCGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAgg                                                        <  1:926717/139‑1 (MQ=255)
                                                                        cGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAggg                                                       <  2:366628/139‑1 (MQ=255)
                                                                         gTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGc                                                      >  1:316278/1‑139 (MQ=255)
                                                                          tAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGAcg                                                                                                                       <  2:622226/73‑1 (MQ=255)
                                                                          tAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGAcg                                                                                                                       >  1:622226/1‑73 (MQ=255)
                                                                           aGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAaga                                                                                       <  1:729383/104‑1 (MQ=255)
                                                                           aGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAaga                                                                                       >  2:729383/1‑104 (MQ=255)
                                                                             cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAaga                                                                                       <  1:38621/102‑1 (MQ=255)
                                                                             cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAaga                                                                                       >  2:38621/1‑102 (MQ=255)
                                                                             cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGc                                                  >  1:383638/1‑139 (MQ=255)
                                                                             cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGc                                                  <  1:1124994/139‑1 (MQ=255)
                                                                             cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGc                                                  >  2:944769/1‑139 (MQ=255)
                                                                               cTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGa                                                >  2:1072521/1‑139 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCTCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                             <  2:585524/139‑1 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTcc                                                                                                           >  1:305114/1‑77 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTcc                                                                                                           <  2:305114/77‑1 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCa                                                                                                     >  2:339983/1‑83 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCa                                                                                                     <  1:339983/83‑1 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAgg                                                                             <  1:273696/107‑1 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAgg                                                                             >  2:273696/1‑107 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                             <  1:70950/139‑1 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                             <  1:288056/139‑1 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                             <  2:383638/139‑1 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                             >  2:143693/1‑139 (MQ=255)
                                                                                  cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                             <  2:316278/139‑1 (MQ=255)
                                                                                   ccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            <  1:214904/139‑1 (MQ=255)
                                                                                   ccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            <  2:149406/139‑1 (MQ=255)
                                                                                   ccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            <  1:459343/139‑1 (MQ=255)
                                                                                   ccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            <  1:143693/139‑1 (MQ=255)
                                                                                     ccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            <  2:294313/137‑1 (MQ=255)
                                                                                     ccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            >  1:294313/1‑137 (MQ=255)
                                                                                       aTGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTgatatcgc                                             <  2:503146/134‑8 (MQ=255)
                                                                                       aTGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTgatatcgc                                             >  1:503146/1‑127 (MQ=255)
                                                                                        tGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAatat                                       >  1:931008/1‑139 (MQ=255)
                                                                                        tGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAatat                                       <  2:966211/139‑1 (MQ=255)
                                                                                          cAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAAtttt                                                                 >  2:757701/1‑111 (MQ=255)
                                                                                          cAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAAtttt                                                                 <  1:757701/111‑1 (MQ=255)
                                                                                          cAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            >  1:734550/1‑132 (MQ=255)
                                                                                          cAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            <  2:734550/132‑1 (MQ=255)
                                                                                             ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTTAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            <  1:667730/129‑1 (MQ=255)
                                                                                             ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTTAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            >  2:667730/1‑129 (MQ=255)
                                                                                             ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGa                                  <  2:682593/139‑1 (MQ=255)
                                                                                             ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGa                                  >  2:313543/1‑139 (MQ=255)
                                                                                             ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGa                                  >  1:1014412/1‑139 (MQ=255)
                                                                                                ggCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAg                               <  2:964533/139‑1 (MQ=255)
                                                                                                   aaTCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTg                            <  1:313543/139‑1 (MQ=255)
                                                                                                                  agaaagGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGaaaa             <  1:783746/139‑1 (MQ=255)
                                                                                                                    aaagGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACt           <  2:248083/139‑1 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCa                                                                                                     >  1:35535/1‑46 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCa                                                                                                     <  2:35535/46‑1 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTTAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTaa                                    <  2:793316/111‑1 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAAtttt                                                                 <  2:397528/82‑1 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAAtttt                                                                 >  1:397528/1‑82 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                             >  1:996720/1‑102 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                             <  2:996720/102‑1 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTaa                                    >  1:793316/1‑111 (MQ=255)
                                                                                                                       ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGt        >  1:743932/1‑139 (MQ=255)
                                                                                                                           tAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTtata    >  1:386355/1‑139 (MQ=255)
                                                                                                                            aGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            <  2:276223/98‑1 (MQ=255)
                                                                                                                            aGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                            >  1:276223/1‑98 (MQ=255)
                                                                                                                            aGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTatat   >  2:84252/1‑139 (MQ=255)
                                                                                                                             gTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATg  >  1:475210/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                     
TACTGAAGCCCTATGTTAATTTCTTTGCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATG  >  NC_000913/2036779‑2037042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: