Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,649	C→T	H311H (CAC→CAT)	yeiH →	UPF0324 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,649	0	C	T	100.0%	241.0 / NA	68	H311H (CAC→CAT)	yeiH	UPF0324 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/32);  total (36/32)

TGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTT  >  NC_000913/2250517‑2250779                                                                                                                                     |                                                                                                                                   tGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAgc                                                                                                                              <  2:124170/139‑1 (MQ=255) tGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAgc                                                                                                                              >  2:725866/1‑139 (MQ=255)    cATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAgcgcg                                                                                                                           <  1:707873/139‑1 (MQ=255)               tCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGc                                                                                                                <  1:725866/139‑1 (MQ=255)                       gCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGa                                                                                                        <  2:722352/139‑1 (MQ=255)                           tCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAAcc                                                                                                    <  2:502034/139‑1 (MQ=255)                                      ttCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATgg                                                                                         <  1:598007/139‑1 (MQ=255)                                      ttCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATgg                                                                                         >  2:185040/1‑139 (MQ=255)                                      ttCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATgg                                                                                         >  1:826310/1‑139 (MQ=255)                                      ttCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATgg                                                                                         <  1:579296/139‑1 (MQ=255)                                      ttCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATgg                                                                                         >  2:83180/1‑139 (MQ=255)                                       tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGc                                                                                                          >  1:201366/1‑121 (MQ=255)                                       tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGc                                                                                                          <  2:201366/121‑1 (MQ=255)                                       tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGc                                                                                        >  2:705542/1‑139 (MQ=255)                                       tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGc                                                                                        >  1:44815/1‑139 (MQ=255)                                        ccACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCa                                                                                       <  2:666107/139‑1 (MQ=255)                                               ttACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTAc                                                                                >  2:788355/1‑139 (MQ=255)                                                tACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCa                                                                                       >  1:565787/1‑131 (MQ=255)                                                tACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCa                                                                                       <  2:565787/131‑1 (MQ=255)                                                 aCCGCAGAGCGTGGTGAATATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTg                                                                              >  2:622953/1‑139 (MQ=255)                                                 aCCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCAc                                                                                      <  2:631702/131‑1 (MQ=255)                                                 aCCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCAc                                                                                      >  1:631702/1‑131 (MQ=255)                                                         gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTg                                                                      >  1:328140/1‑139 (MQ=255)                                                         gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTg                                                                      >  1:960887/1‑139 (MQ=255)                                                          cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCCATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGc                                                                         >  1:734715/1‑135 (MQ=255)                                                          cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCt                                                                                                               >  2:382367/1‑97 (MQ=255)                                                          cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCt                                                                                                               <  1:382367/97‑1 (MQ=255)                                                          cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGc                                                                         >  1:249252/1‑135 (MQ=255)                                                          cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGc                                                                         <  2:249252/135‑1 (MQ=255)                                                          cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTgg                                                                     <  1:83180/139‑1 (MQ=255)                                                          cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTgg                                                                     >  1:636671/1‑139 (MQ=255)                                                          cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACCCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGc                                                                         <  2:734715/135‑1 (MQ=255)                                                             ggtgAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGTGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTg                                                                  >  2:860758/1‑139 (MQ=255)                                                              gtgAACATGCTGGTAACGCTGGATACCTTCCTGCTGTCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGa                                                                 <  1:705542/139‑1 (MQ=255)                                                                  aCATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCt                                                                   <  1:97138/133‑1 (MQ=255)                                                                  aCATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCt                                                                   >  2:97138/1‑133 (MQ=255)                                                                         ggTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggt                                                      >  1:239744/1‑139 (MQ=255)                                                                          gTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtg                                                     <  1:415458/139‑1 (MQ=255)                                                                           tAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCt                                                                   <  2:348259/124‑1 (MQ=255)                                                                           tAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCt                                                                   >  1:348259/1‑124 (MQ=255)                                                                           tAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtgg                                                    >  2:970152/1‑139 (MQ=255)                                                                            aaCGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTggtggtggt                                                   <  1:185040/139‑1 (MQ=255)                                                                            aaCGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTggtggtggt                                                   <  2:826310/139‑1 (MQ=255)                                                                               gCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                <  2:253955/139‑1 (MQ=255)                                                                                 tGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                              >  2:227411/1‑139 (MQ=255)                                                                                 tGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                              >  1:75500/1‑139 (MQ=255)                                                                                   gATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTa                                            >  2:638015/1‑139 (MQ=255)                                                                                    aTACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGc                                                 >  1:226814/1‑133 (MQ=255)                                                                                    aTACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGc                                                 <  2:226814/133‑1 (MQ=255)                                                                                        cTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAt                                       >  1:492839/1‑139 (MQ=255)                                                                                             tgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAt                                  >  1:153602/1‑139 (MQ=255)                                                                                             tgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAt                                  >  1:393265/1‑139 (MQ=255)                                                                                                  gCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtgg                                                          >  2:407096/1‑110 (MQ=255)                                                                                                  gCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtgg                                                          <  1:407096/110‑1 (MQ=255)                                                                                                  gCAATGGCGATGGCGGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCaaa                             >  1:839643/1‑139 (MQ=255)                                                                                                      tggcgatggcgGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGc                                                 <  2:599257/115‑1 (MQ=255)                                                                                                      tggcgatggcgGCGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGc                                                 >  1:599257/1‑115 (MQ=255)                                                                                                             ggcggcGCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTTGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCa                  <  2:174030/139‑1 (MQ=255)                                                                                                               cggcgCTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATa                <  1:788355/139‑1 (MQ=255)                                                                                                                  cgcTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCa                                                                                       <  1:717063/65‑1 (MQ=255)                                                                                                                  cgcTGGGTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCa                                                                                       >  2:717063/1‑65 (MQ=255)                                                                                                                      gggTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACt         <  2:839643/139‑1 (MQ=255)                                                                                                                       ggTCTGACCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTa        <  1:738211/139‑1 (MQ=255)                                                                                                                             aCCACTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCtt  >  2:822680/1‑139 (MQ=255)                                                                                                                                 cTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtg                                                     >  1:356001/1‑84 (MQ=255)                                                                                                                                 cTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtg                                                     <  2:356001/84‑1 (MQ=255)                                                                                                                                 cTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTggtggtggt                                                   <  1:196903/86‑1 (MQ=255)                                                                                                                                 cTCATGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTggtggtggt                                                   >  2:196903/1‑86 (MQ=255)                                                                                                                                     |                                                                                                                                   TGCCATCTTGTTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTT  >  NC_000913/2250517‑2250779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑