Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,459,283	G→A	A396V (GCG→GTG)	fadI ←	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,459,283	0	G	A	100.0%	129.9 / NA	37	A396V (GCG→GTG)	fadI	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/20);  total (17/20)

GTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGA  >  NC_000913/2459149‑2459408                                                                                                                                       |                                                                                                                              gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcaccgc                                                                                                                           <  2:752082/139‑1 (MQ=255) gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcaccgc                                                                                                                           <  2:841962/139‑1 (MQ=255)        ccATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGa                                                                                                                    <  1:682938/139‑1 (MQ=255)        ccATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGa                                                                                                                    <  2:973190/139‑1 (MQ=255)              ttCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCg                                                                                                              >  1:840689/1‑139 (MQ=255)                  gCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAg                                                                                                          <  2:719297/139‑1 (MQ=255)                   ccTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGc                                                                                                         >  1:896829/1‑139 (MQ=255)                         gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGa                                                                                                   <  1:610589/139‑1 (MQ=255)                                     cgcCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCAc                                                                                       >  2:1012691/1‑139 (MQ=255)                                        cAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGtt                                                                                    <  1:413014/139‑1 (MQ=255)                                        cAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGtt                                                                                    <  1:617/139‑1 (MQ=255)                                        cAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGtt                                                                                    <  1:618117/139‑1 (MQ=255)                                          aGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTaa                                                                                  >  2:6274/1‑139 (MQ=255)                                                  ggCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTa                                                                          <  1:267541/139‑1 (MQ=255)                                                          aGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCAc                                                                  <  2:48431/139‑1 (MQ=255)                                                                 aaCTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCa                                                           >  2:193870/1‑139 (MQ=255)                                                                   cTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGt                                                         <  1:325999/139‑1 (MQ=255)                                                                      aaCCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGc                                                      <  1:1012691/139‑1 (MQ=255)                                                                            aTCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCg                                                                                                    <  2:623772/87‑1 (MQ=255)                                                                            aTCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCg                                                                                                    >  1:623772/1‑87 (MQ=255)                                                                            aTCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGc                                                >  1:660981/1‑139 (MQ=255)                                                                                     cgcgACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAg                                       >  1:306064/1‑139 (MQ=255)                                                                                       cgACGGCAAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTg                                     >  2:736207/1‑139 (MQ=255)                                                                                            gCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCa                                >  2:122382/1‑139 (MQ=255)                                                                                                      tGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAAc                      <  1:59549/139‑1 (MQ=255)                                                                                                                 ggTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTAccc           <  2:325501/139‑1 (MQ=255)                                                                                                                  gTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGc                                                      <  1:566188/95‑1 (MQ=255)                                                                                                                  gTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGc                                                      >  2:566188/1‑95 (MQ=255)                                                                                                                     atcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCg                                                                                                    >  2:467629/1‑46 (MQ=255)                                                                                                                     atcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCg                                                                                                    <  1:467629/46‑1 (MQ=255)                                                                                                                     atcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCa       <  1:122382/139‑1 (MQ=255)                                                                                                                     atcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCa       >  2:189547/1‑139 (MQ=255)                                                                                                                      tcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCaa      >  2:444597/1‑139 (MQ=255)                                                                                                                       catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGaa                                                                                                  <  2:589102/46‑1 (MQ=255)                                                                                                                       catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGaa                                                                                                  >  1:589102/1‑46 (MQ=255)                                                                                                                       catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGc        >  2:505167/1‑136 (MQ=255)                                                                                                                       catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGc        <  1:505167/136‑1 (MQ=255)                                                                                                                       catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAAc     >  1:968110/1‑139 (MQ=255)                                                                                                                          ccGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGa  <  2:925672/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                              GTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGA  >  NC_000913/2459149‑2459408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑