Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,546,932 C→T P87L (CCG→CTG)  murP → N‑acetylmuramic acid permease, EIIBC component, PTS system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,546,9320CT100.0% 86.7 / NA 26P87L (CCG→CTG) murPN‑acetylmuramic acid permease, EIIBC component, PTS system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

GATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGG  >  NC_000913/2546802‑2547067
                                                                                                                                  |                                                                                                                                       
gATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGATGCACTGGTACAgg                                                                                                                                 >  1:857835/1‑139 (MQ=255)
gATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAgg                                                                                                                                 >  1:47360/1‑139 (MQ=255)
       atatCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGc                                                                                                                          >  2:574427/1‑139 (MQ=255)
               aCGCTGGAAGGCGTGAAGGGCGTCATTTTGGCCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGa                                                                                                                       >  2:10808/1‑134 (MQ=255)
               aCGCTGGAAGGCGTGAAGGGCGTCATTTTGGCCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGa                                                                                                                       <  1:10808/134‑1 (MQ=255)
               aCGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATcg                                                                                                                  <  1:1093811/139‑1 (MQ=255)
                 gCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATcgcc                                                                                                                <  1:574427/139‑1 (MQ=255)
                     gAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGccc                                                                                                            >  2:582029/1‑139 (MQ=255)
                           gTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACa                                                                                                      <  1:403893/139‑1 (MQ=255)
                                gggCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGt                                                                                                 <  1:426770/139‑1 (MQ=255)
                                           tGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGc                                                                                      <  1:1024938/139‑1 (MQ=255)
                                             aCCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATcg                                                                                                                  >  1:2536/1‑109 (MQ=255)
                                             aCCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATcg                                                                                                                  <  2:2536/109‑1 (MQ=255)
                                                            cAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCg                                                                     <  2:544459/139‑1 (MQ=255)
                                                               gTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGa                                                                                                                       <  1:1022406/86‑1 (MQ=255)
                                                               gTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGa                                                                                                                       >  2:1022406/1‑86 (MQ=255)
                                                                   tttttGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGcaaca                                                              >  2:784198/1‑139 (MQ=255)
                                                                              ggTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAAttt                                                          >  2:972796/1‑132 (MQ=255)
                                                                              ggTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAAttt                                                          <  1:972796/132‑1 (MQ=255)
                                                                                         tCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCg                                                                     >  2:1048911/1‑110 (MQ=255)
                                                                                         tCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCg                                                                     <  1:1048911/110‑1 (MQ=255)
                                                                                              ccGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCtt                                   >  1:295555/1‑139 (MQ=255)
                                                                                                     aGCGATGAGCGAGCTGCTGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCAcgccg                            <  1:1025868/139‑1 (MQ=255)
                                                                                                                 gctgctGGGAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGt                >  2:481983/1‑139 (MQ=255)
                                                                                                                       gggAGAAGCACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTAtt          >  1:1026101/1‑139 (MQ=255)
                                                                                                                               cACTGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCgg  >  1:71520/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                       
GATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGG  >  NC_000913/2546802‑2547067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: