Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,720,122	T→C	V57A (GTG→GCG)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,720,122	0	T	C	100.0%	134.5 / NA	36	V57A (GTG→GCG)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/20);  total (16/20)

AATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAACCTGCATTAT  >  NC_000913/2719990‑2720248                                                                                                                                     |                                                                                                                               aaTCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggt                                                                                                                          <  1:1028437/139‑2 (MQ=255) aaTCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggc                                                                                                                          <  2:610077/139‑1 (MQ=255) aaTCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggc                                                                                                                          <  1:1005379/139‑1 (MQ=255)               aTTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTg                                                                                                            <  2:244623/139‑1 (MQ=255)                   gcgcGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggcc                                                                                                                         >  2:705241/1‑122 (MQ=255)                   gcgcGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggcc                                                                                                                         <  1:705241/122‑1 (MQ=255)                   gcgcGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAg                                                                                                        >  1:124184/1‑139 (MQ=255)                    cgcgTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGAGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGc                                                                                                       <  2:707261/139‑1 (MQ=255)                       gTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCCGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCCGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTg                                                                                                    >  2:824963/1‑139 (MQ=255)                        tCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGc                                                                                                   >  2:530087/1‑139 (MQ=255)                                          gcAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTg                                                                                 <  2:295013/139‑1 (MQ=255)                                            aGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCg                                                                               <  1:912616/139‑1 (MQ=255)                                            aGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCg                                                                               <  1:272305/139‑1 (MQ=255)                                               ttACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGtt                                                                            <  1:288262/139‑1 (MQ=255)                                                  ccTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTa                                                                         <  1:911049/139‑1 (MQ=255)                                                        gatgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTAcc                                                                   >  2:259074/1‑139 (MQ=255)                                                        gatgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTAcc                                                                   >  1:511725/1‑139 (MQ=255)                                                            cGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATg                                                               >  2:851454/1‑139 (MQ=255)                                                             gTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGc                                                              <  2:124184/139‑1 (MQ=255)                                                                    gctgGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCt                                                                                   <  2:974618/111‑1 (MQ=255)                                                                    gctgGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCt                                                                                   >  1:974618/1‑111 (MQ=255)                                                                     ctgGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTa                                                      >  2:412289/1‑139 (MQ=255)                                                                        gCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATc                                                   >  1:492780/1‑139 (MQ=255)                                                                           ggAGGCTTTGACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTg                                                <  2:662152/139‑1 (MQ=255)                                                                                         aCCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAagag                                  <  1:851454/139‑1 (MQ=255)                                                                                                  cccGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCgag                         <  1:259074/139‑1 (MQ=255)                                                                                                   ccGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCgaga                        >  1:204546/1‑139 (MQ=255)                                                                                                     ggTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGaaa                      >  1:55367/1‑139 (MQ=255)                                                                                                       tGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAgg                    <  1:894791/139‑1 (MQ=255)                                                                                                        gACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGc                   >  1:521409/1‑139 (MQ=255)                                                                                                           gCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCtt                                                                                                     >  2:1065868/1‑54 (MQ=255)                                                                                                           gCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCtt                                                                                                     <  1:1065868/54‑1 (MQ=255)                                                                                                             cAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTaa              >  1:416186/1‑139 (MQ=255)                                                                                                                ccTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAAc           <  2:511725/139‑1 (MQ=255)                                                                                                                ccTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAAc           <  1:824963/139‑1 (MQ=255)                                                                                                                         cGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAACCTGCattat  >  2:1120983/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                               AATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAACCTGCATTAT  >  NC_000913/2719990‑2720248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑