Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,920,310	A→G	V146A (GTC→GCC)	gudX ←	glucarate dehydratase‑related protein, substrate unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,920,310	0	A	G	100.0%	163.4 / NA	46	V146A (GTC→GCC)	gudX	glucarate dehydratase‑related protein, substrate unknown
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/20);  total (26/20)

ACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAGGCAC  >  NC_000913/2920184‑2920445                                                                                                                               |                                                                                                                                        acaACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCt                                                                                                                             >  2:712931/1‑139 (MQ=255)            gCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgcttg                                                                                                                  >  1:710495/1‑139 (MQ=255)             cTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgcttgc                                                                                                                 <  2:1083790/139‑1 (MQ=255)               gTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCt                                                                                                               <  1:577315/139‑1 (MQ=255)               gTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCt                                                                                                               <  2:875541/139‑1 (MQ=255)                          tCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgcttgc                                                                                                                 <  1:877646/126‑1 (MQ=255)                          tCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgcttgc                                                                                                                 >  2:877646/1‑126 (MQ=255)                                          aTACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTaa                                                                                                                                   >  1:601839/1‑92 (MQ=255)                                          aTACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTaa                                                                                                                                   <  2:601839/92‑1 (MQ=255)                                             ccACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTc                                                                                 <  1:291530/139‑1 (MQ=255)                                                 tCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAgcg                                                                             >  1:456923/1‑139 (MQ=255)                                                        tGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCt                                                                                                         <  1:259222/104‑1 (MQ=255)                                                        tGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCt                                                                                                         >  2:259222/1‑104 (MQ=255)                                                          cccGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTa                                                                    >  1:235723/1‑139 (MQ=255)                                                                    tttCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCg                                                                                                >  1:550831/1‑101 (MQ=255)                                                                    tttCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCg                                                                                                <  2:550831/101‑1 (MQ=255)                                                                                    aTCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAgc                                          >  1:55085/1‑139 (MQ=255)                                                                                    aTCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAgc                                          >  2:754073/1‑139 (MQ=255)                                                                                        gTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGcgccg                                      >  2:518220/1‑139 (MQ=255)                                                                                         ttttGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAgcg                                                                             <  1:501965/99‑1 (MQ=255)                                                                                         ttttGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAgcg                                                                             >  2:501965/1‑99 (MQ=255)                                                                                           ttGGTCCGATCACCGATATAAAACAGATAACCGAGGGGGGTAAGAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCg                                                                                                >  1:751244/1‑78 (MQ=255)                                                                                           ttGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGt                                                                                                   <  1:18361/75‑1 (MQ=255)                                                                                           ttGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGt                                                                                                   >  2:18361/1‑75 (MQ=255)                                                                                           ttGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCg                                                                                                <  2:751244/78‑1 (MQ=255)                                                                                           ttGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCa                                   <  1:794774/139‑1 (MQ=255)                                                                                           ttGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCa                                   <  1:489519/139‑1 (MQ=255)                                                                                           ttGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTAGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCa                                   >  2:794774/1‑139 (MQ=255)                                                                                              gTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgc                                                                                                                     >  2:232774/1‑54 (MQ=255)                                                                                              gTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgc                                                                                                                     <  1:232774/54‑1 (MQ=255)                                                                                              gTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGa                                                                                            >  2:173064/1‑79 (MQ=255)                                                                                              gTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGa                                                                                            <  1:173064/79‑1 (MQ=255)                                                                                              gTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACgg                                <  2:710495/139‑1 (MQ=255)                                                                                                   aTCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTa                           >  2:984851/1‑139 (MQ=255)                                                                                                   aTCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTa                           >  2:169920/1‑139 (MQ=255)                                                                                                   aTCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTa                           >  1:87373/1‑139 (MQ=255)                                                                                                               aaaCAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCa               <  2:235723/139‑1 (MQ=255)                                                                                                                     aTAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTc         >  2:642460/1‑139 (MQ=255)                                                                                                                     aTAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTc         >  2:1091886/1‑139 (MQ=255)                                                                                                                     aTAACCGAGGGCGGTAATAGCCTCGCGTTGCCTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCa               >  2:552640/1‑133 (MQ=255)                                                                                                                     aTAACCGAGGGCGGTAATAGCCTCGCGTTGCCTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCa               <  1:552640/133‑1 (MQ=255)                                                                                                                       aaCCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAAGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCa       >  2:149787/1‑139 (MQ=255)                                                                                                                         ccGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAgg     <  1:642460/139‑1 (MQ=255)                                                                                                                           gAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAGGCa   >  2:354220/1‑139 (MQ=255)                                                                                                                            aGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAGGCAc  <  2:456923/139‑1 (MQ=255)                                                                                                                            aGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAGGCAc  >  2:170042/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                        ACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAGGCAC  >  NC_000913/2920184‑2920445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑