Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,965,748	T→C	D97G (GAC→GGC)	lgt ←	phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,965,748	0	T	C	100.0%	174.0 / NA	49	D97G (GAC→GGC)	lgt	phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

ACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTG  >  NC_000913/2965618‑2965879                                                                                                                                   |                                                                                                                                    aCCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACGACGATAACGGCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGaca                                                                                                                             <  2:692978/139‑1 (MQ=255)  ccAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGacac                                                                                                                            <  1:93171/139‑1 (MQ=255)    aaGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACgg                                                                                                                          <  2:325662/139‑1 (MQ=255)                  tGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGTCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCgg                                                                                                            >  2:74468/1‑139 (MQ=255)                  tGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCgg                                                                                                            >  1:64924/1‑139 (MQ=255)                        gtgCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCAAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACCGAACAGATACACCGGATCggt                                                                                                      >  2:657092/1‑138 (MQ=255)                          gCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCa                                                                                                    >  2:996950/1‑139 (MQ=255)                          gCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCa                                                                                                    >  2:134917/1‑139 (MQ=255)                           cGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCAt                                                                                                   >  2:405936/1‑139 (MQ=255)                              tAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa                                                                                                >  2:337339/1‑139 (MQ=255)                                      gagaCCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGTCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGa                                                                                        <  1:74468/139‑1 (MQ=255)                                       agaCCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGaa                                                                                       >  2:687081/1‑139 (MQ=255)                                        gaCCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGaaa                                                                                      <  1:337339/139‑1 (MQ=255)                                            tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa                                                                                                >  2:946341/1‑125 (MQ=255)                                            tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa                                                                                                <  1:946341/125‑1 (MQ=255)                                            tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGt                                                                                  >  1:124917/1‑139 (MQ=255)                                            tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGt                                                                                  >  1:514152/1‑139 (MQ=255)                                             ggAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGa                                                                                        >  2:533542/1‑132 (MQ=255)                                             ggAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGa                                                                                        <  1:533542/132‑1 (MQ=255)                                                agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaa                                                                              <  2:514152/139‑1 (MQ=255)                                                 gaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaac                                                                             <  1:62122/139‑1 (MQ=255)                                                     gAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaa                                                                         <  1:990710/139‑1 (MQ=255)                                                        cGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACAt                                                                      <  1:687081/139‑1 (MQ=255)                                                            tAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAAcc                                                                  <  2:181969/139‑1 (MQ=255)                                                            tAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAAcc                                                                  <  1:405936/139‑1 (MQ=255)                                                                  ggcgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACg                                                            <  1:499963/139‑1 (MQ=255)                                                                    cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGacac                                                                                                                            <  1:140035/73‑1 (MQ=255)                                                                    cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGacac                                                                                                                            >  2:140035/1‑73 (MQ=255)                                                                    cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaac                                                                             <  2:769702/120‑1 (MQ=255)                                                                    cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaac                                                                             >  1:769702/1‑120 (MQ=255)                                                                            gATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAgg                                                  <  2:64924/139‑1 (MQ=255)                                                                                  catcaCCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACg                                            <  2:1096310/139‑1 (MQ=255)                                                                                       ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGt                                                           <  2:65464/119‑1 (MQ=255)                                                                                       ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGt                                                           >  1:65464/1‑119 (MQ=255)                                                                                       ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGa                                              >  2:447950/1‑132 (MQ=255)                                                                                       ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGa                                              <  1:447950/132‑1 (MQ=255)                                                                                         acGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGa                                     <  1:522750/139‑1 (MQ=255)                                                                                           gATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAg                                   >  1:822368/1‑139 (MQ=255)                                                                                               aCGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCg                               <  2:329691/139‑1 (MQ=255)                                                                                                cGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGc                              <  2:1047176/139‑1 (MQ=255)                                                                                                 gCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCa                             <  2:305118/139‑1 (MQ=255)                                                                                                 gCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCa                             <  1:49046/139‑1 (MQ=255)                                                                                                  ccAATCAGGCCGCCGTGGAAAGTCATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaac                                                                        <  1:952171/95‑1 (MQ=255)                                                                                                  ccAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaac                                                                        >  2:952171/1‑95 (MQ=255)                                                                                                          gccgccGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAg                    >  1:274132/1‑139 (MQ=255)                                                                                                             gccgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGttt                 >  2:1028489/1‑139 (MQ=255)                                                                                                               cgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGa                                     >  1:812396/1‑117 (MQ=255)                                                                                                               cgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGa                                     <  2:812396/117‑1 (MQ=255)                                                                                                                     aaGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTc         <  1:996950/139‑1 (MQ=255)                                                                                                                            gccgccgccCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCg                               <  1:109775/110‑1 (MQ=255)                                                                                                                            gccgccgccCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCg                               >  2:109775/1‑110 (MQ=255)                                                                                                                            gccgccgccCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTg  >  2:989902/1‑139 (MQ=255)                                                                                                                              cgccgcCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGc                                  <  1:154964/105‑1 (MQ=255)                                                                                                                              cgccgcCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGc                                  >  2:154964/1‑105 (MQ=255)                                                                                                                                   |                                                                                                                                    ACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTG  >  NC_000913/2965618‑2965879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑