Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,067,677	G→A	A135V (GCG→GTG)	yggE ←	oxidative stress defense protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,067,677	0	G	A	100.0%	198.3 / NA	57	A135V (GCG→GTG)	yggE	oxidative stress defense protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/32);  total (25/32)

CGATGAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCGCCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATCTGGCTGGGTGCGTA  >  NC_000913/3067548‑3067809                                                                                                                                  |                                                                                                                                     cGATGAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAgcg                                                                                                                             <  1:671377/139‑1 (MQ=255)   aTGAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAgcgcg                                                                                                                           <  2:446062/139‑1 (MQ=255)   aTGAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAgcgcg                                                                                                                           <  1:431571/139‑1 (MQ=255)     gAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGcc                                                                                                                         <  1:341591/139‑1 (MQ=255)       aaGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCAt                                                                                                                       >  2:306496/1‑139 (MQ=255)        aGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATc                                                                                                                      <  1:67059/139‑1 (MQ=255)                     ttCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAgcgcg                                                                                                                           >  2:23384/1‑121 (MQ=255)                     ttCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAgcgcg                                                                                                                           <  1:23384/121‑1 (MQ=255)                                    aaTCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGa                                                                                          <  2:113786/139‑1 (MQ=255)                                        gcgTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGcc                                                                                                                         >  2:600884/1‑104 (MQ=255)                                        gcgTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGcc                                                                                                                         <  1:600884/104‑1 (MQ=255)                                            tATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGt                                                                                  >  1:615851/1‑139 (MQ=255)                                            tATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGt                                                                                  <  2:398797/139‑1 (MQ=255)                                                             cgcgCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTca                                                                 >  2:901652/1‑139 (MQ=255)                                                                  tttGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAgcgcg                                                                                                                           >  2:564929/1‑76 (MQ=255)                                                                  tttGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAgcgcg                                                                                                                           <  1:564929/76‑1 (MQ=255)                                                                  tttGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCa                                                                                                                 >  1:961916/1‑86 (MQ=255)                                                                  tttGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCa                                                                                                                 <  2:961916/86‑1 (MQ=255)                                                                  tttGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAgcg                                                            <  2:181992/139‑1 (MQ=255)                                                                      tCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGt                                                        <  1:602506/139‑1 (MQ=255)                                                                            taGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGc                                                                                    <  1:455554/105‑1 (MQ=255)                                                                            taGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGc                                                                                    >  2:455554/1‑105 (MQ=255)                                                                            taGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCtt                                                  >  2:138324/1‑139 (MQ=255)                                                                               gCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTaa                                               >  2:1098298/1‑139 (MQ=255)                                                                                cATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAg                                              >  2:974678/1‑139 (MQ=255)                                                                                cATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAg                                              >  1:1036871/1‑139 (MQ=255)                                                                                 aTCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAgg                                             <  2:530615/139‑1 (MQ=255)                                                                                 aTCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAgg                                             <  1:538058/139‑1 (MQ=255)                                                                                  tCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGc                                                                                    <  1:88899/99‑1 (MQ=255)                                                                                  tCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGc                                                                                    >  2:88899/1‑99 (MQ=255)                                                                                  tCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGa                                            >  2:986119/1‑139 (MQ=255)                                                                                  tCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGa                                            <  2:565286/139‑1 (MQ=255)                                                                                  tCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGa                                            <  1:231285/139‑1 (MQ=255)                                                                                  tCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGa                                            >  2:355215/1‑139 (MQ=255)                                                                                  tCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGa                                            >  2:231285/1‑139 (MQ=255)                                                                                  tCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGa                                            >  1:897580/1‑139 (MQ=255)                                                                                    cGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGt                                                        <  1:679976/125‑1 (MQ=255)                                                                                    cGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATa                                          >  1:1033836/1‑139 (MQ=255)                                                                                    cGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATa                                          >  1:233162/1‑139 (MQ=255)                                                                                    cGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTATGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGt                                                        >  2:679976/1‑125 (MQ=255)                                                                                          cgcCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTa                                                       <  2:640080/120‑1 (MQ=255)                                                                                          cgcCACGCCCAGCGACACAGAACGAATTTCCTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCGAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTa                                                       >  1:640080/1‑120 (MQ=255)                                                                                           gcCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTAc                                   <  2:233162/139‑1 (MQ=255)                                                                                                   cAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGa                           <  1:121675/139‑1 (MQ=255)                                                                                                   cAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGa                           <  2:1033836/139‑1 (MQ=255)                                                                                                   cAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGa                           <  1:672459/139‑1 (MQ=255)                                                                                                      cGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTcc                                                                           <  1:267264/88‑1 (MQ=255)                                                                                                      cGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTcc                                                                           >  2:267264/1‑88 (MQ=255)                                                                                                       gACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGataat                       <  1:782201/139‑1 (MQ=255)                                                                                                           caGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGataatcata                   <  2:615851/139‑1 (MQ=255)                                                                                                                 gAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATctgg             <  2:306267/139‑1 (MQ=255)                                                                                                                 gAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATctgg             <  1:1098298/139‑1 (MQ=255)                                                                                                                     ttCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATctggctgg         <  1:974678/139‑1 (MQ=255)                                                                                                                         ttAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTcc                                                                           >  2:765400/1‑69 (MQ=255)                                                                                                                         ttAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTcc                                                                           <  1:765400/69‑1 (MQ=255)                                                                                                                         ttAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATCTGGCTGGGTGc     >  2:661346/1‑139 (MQ=255)                                                                                                                            aGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATCTGGCTGGGTGCGTa  >  2:533450/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                     CGATGAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCGCCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATCTGGCTGGGTGCGTA  >  NC_000913/3067548‑3067809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑