Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,004	A→G	G114G (GGT→GGC)	tdh ←	L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,004	0	A	G	100.0%	238.6 / NA	63	G114G (GGT→GGC)	tdh	L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/32);  total (31/32)

ACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATAC  >  NC_000913/3790868‑3791134                                                                                                                                         |                                                                                                                                   aCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc                                                                                                                                  >  1:611981/1‑139 (MQ=255)   gACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCaa                                                                                                                                >  1:811604/1‑139 (MQ=255)    aCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAAc                                                                                                                               <  2:811604/139‑1 (MQ=255)    aCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAAc                                                                                                                               <  2:31160/139‑1 (MQ=255)      aGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGc                                                                                                                             >  1:12240/1‑139 (MQ=255)                    ggCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCgg                                                                                                               <  1:529355/139‑1 (MQ=255)                    ggCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCgg                                                                                                               <  2:291863/139‑1 (MQ=255)                         tACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa                                                                                                          >  2:1003473/1‑139 (MQ=255)                          aCCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaaa                                                                                                         >  1:574728/1‑139 (MQ=255)                           ccGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGtt                                                                                                                   <  1:770451/128‑1 (MQ=255)                           ccGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGtt                                                                                                                   >  2:770451/1‑128 (MQ=255)                            cGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATg                                                                                                       <  2:611981/139‑1 (MQ=255)                                gggATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTa                                                                                                   >  1:120220/1‑139 (MQ=255)                                  gATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACg                                                                                                 >  1:788348/1‑139 (MQ=255)                                   aTCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGa                                                                                                >  1:256429/1‑139 (MQ=255)                                              cGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACa                                                                                     >  1:261826/1‑139 (MQ=255)                                              cGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACa                                                                                     >  1:93786/1‑139 (MQ=255)                                                       cATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa                                                                            <  2:256429/139‑1 (MQ=255)                                                        aTCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCaa                                                                           <  1:290063/139‑1 (MQ=255)                                                        aTCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCaa                                                                           <  1:1003473/139‑1 (MQ=255)                                                          cGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATg                                                                         >  2:88465/1‑139 (MQ=255)                                                               atatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACcac                                                                    <  2:12240/139‑1 (MQ=255)                                                                tatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCGGATATTCGGGAAAGCAGCCCGGGCGATTAACGCAAACGCCAATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACcaaa                                                                   >  2:692098/1‑137 (MQ=255)                                                                tatTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACcaca                                                                   <  1:133634/139‑1 (MQ=255)                                                                 atTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                  <  1:833909/139‑1 (MQ=255)                                                                 atTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGACACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                  <  1:1027378/139‑1 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTTCGGCACCAATGGGTACGACCACCACGACAGTTGCGGCAACGACCACAGGTGata                                                            >  2:949581/1‑139 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                  <  1:234745/133‑1 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                  >  2:234745/1‑133 (MQ=255)                                                                       ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGata                                                            >  2:64409/1‑139 (MQ=255)                                                                            ttttGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGcc                                                       <  2:77139/139‑1 (MQ=255)                                                                                     cGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGa                                              <  2:719097/139‑1 (MQ=255)                                                                                       ttGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGaaa                                            >  1:338317/1‑139 (MQ=255)                                                                                         gAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAAcg                                          >  1:412834/1‑139 (MQ=255)                                                                                             gCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGAt                                      <  2:338317/139‑1 (MQ=255)                                                                                                 ggATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGcc                                  <  2:344577/139‑1 (MQ=255)                                                                                                 ggATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGcc                                  >  2:332631/1‑139 (MQ=255)                                                                                                      aCCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCt                             >  2:163163/1‑139 (MQ=255)                                                                                                        cAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTg                           <  1:88465/139‑1 (MQ=255)                                                                                                           atatTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAg                        >  2:30381/1‑139 (MQ=255)                                                                                                            tatTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGc                       <  2:781464/139‑1 (MQ=255)                                                                                                            tatTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGc                       <  1:46672/139‑1 (MQ=255)                                                                                                             atTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGcc                      >  1:583392/1‑139 (MQ=255)                                                                                                               tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAg                        <  2:645473/135‑1 (MQ=255)                                                                                                               tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAg                        >  1:645473/1‑135 (MQ=255)                                                                                                               tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCATGTTGCGGCACAAATGGGTACGACCACCACGCCAGTTGCCGCAATGACAACAGGTGATATGGCCTTCGCAAGAAACGCGATCGCCGATCTTGAAGCCtt                    >  2:393703/1‑139 (MQ=255)                                                                                                                 ggCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTc                  >  2:615185/1‑139 (MQ=255)                                                                                                                   gcaaGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGA‑CTTGAAGCCTTTCACt               >  1:496544/3‑139 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca                                                                                                             >  1:673539/1‑43 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca                                                                                                             <  2:673539/43‑1 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacc                                                                                              >  1:1010739/1‑58 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacc                                                                                              <  2:1010739/58‑1 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                  <  1:487414/86‑1 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAg                                                                  >  2:487414/1‑86 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTc             <  2:574728/139‑1 (MQ=255)                                                                                                                      agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTc             >  1:8855/1‑139 (MQ=255)                                                                                                                           cccGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc        <  1:460661/139‑1 (MQ=255)                                                                                                                           cccGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc        <  1:393428/139‑1 (MQ=255)                                                                                                                           cccGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc        <  1:170512/139‑1 (MQ=255)                                                                                                                             cGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCa      <  2:120220/139‑1 (MQ=255)                                                                                                                             cGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCa      <  1:64409/139‑1 (MQ=255)                                                                                                                             cGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCa      <  2:788348/139‑1 (MQ=255)                                                                                                                                 cGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAtac  >  2:312161/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                   ACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATAC  >  NC_000913/3790868‑3791134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑