| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,901,765 | G→A | G152G (GGC→GGT) | bglH ← | carbohydrate‑specific outer membrane porin, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,901,765 | 0 | G | A | 98.5% | 227.7 / NA | 66 | G152G (GGC→GGT) | bglH | carbohydrate‑specific outer membrane porin, cryptic |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (31/34); minor base C (0/1); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGA > NC_000913/3901631‑3901899 | aaCCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCa < 2:673196/139‑1 (MQ=255) aCCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCag < 2:386458/139‑1 (MQ=255) aGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAgaga > 1:214832/1‑139 (MQ=255) gtcctgctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGGCACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTAGTTACCAAAGCGCCCCAGAGAACCAATCGcc < 2:1082438/136‑1 (MQ=255) gTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc < 1:108104/139‑1 (MQ=255) gTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc < 2:687483/139‑1 (MQ=255) gTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc < 2:491416/139‑1 (MQ=255) gTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc < 2:134212/139‑1 (MQ=255) gTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc < 1:908714/139‑1 (MQ=255) ctgctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCAt > 1:844521/1‑139 (MQ=255) ctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGAt > 1:214098/1‑139 (MQ=255) aCCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTa > 2:1052890/1‑139 (MQ=255) cAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAgg < 2:461082/139‑1 (MQ=255) cAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAgg < 2:284526/139‑1 (MQ=255) cGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGa < 1:224367/139‑1 (MQ=255) gccatcATGAAGACGGCATCATCCAGTTTGCCAATTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAGACCAGCCGGAGTATTCGTAACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgag < 2:844521/138‑1 (MQ=255) ccatcATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgaga < 1:826650/139‑1 (MQ=255) catcatCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCaa < 2:938469/110‑1 (MQ=255) catcatCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCaa > 1:938469/1‑110 (MQ=255) catcatCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGagag > 2:965376/1‑139 (MQ=255) catcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg < 2:50043/139‑1 (MQ=255) catcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg < 1:965376/139‑1 (MQ=255) tcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGt > 2:969496/1‑139 (MQ=255) tcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGt < 2:290217/139‑1 (MQ=255) caACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTccc < 1:164111/139‑1 (MQ=255) caACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTccc > 1:766322/1‑139 (MQ=255) gCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAc < 1:1052890/139‑1 (MQ=255) cATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAcc > 1:37772/1‑139 (MQ=255) cATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAcc > 2:373019/1‑139 (MQ=255) cATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAcc > 2:329999/1‑139 (MQ=255) tCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAGGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAg < 2:268822/139‑1 (MQ=255) tCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg < 1:912425/126‑1 (MQ=255) tCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg > 2:912425/1‑126 (MQ=255) aaCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGaa < 1:373019/139‑1 (MQ=255) aCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg > 1:1118176/1‑123 (MQ=255) aCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg < 2:1118176/123‑1 (MQ=255) aCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg > 1:181526/1‑123 (MQ=255) aCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg < 2:181526/123‑1 (MQ=255) aCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAAc > 1:41921/1‑139 (MQ=255) cGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGt < 2:214098/139‑1 (MQ=255) gCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAg < 2:9700/139‑1 (MQ=255) ttttCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGt > 2:5455/1‑139 (MQ=255) tttCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAg > 1:355910/1‑134 (MQ=255) tttCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAg < 2:355910/134‑1 (MQ=255) gttgttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg < 2:421556/95‑1 (MQ=255) gttgttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCg > 1:421556/1‑95 (MQ=255) gttgttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAt > 1:551637/1‑139 (MQ=255) gttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCACTCGccc > 1:96065/1‑66 (MQ=255) gttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGccc < 2:96065/66‑1 (MQ=255) aaCTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAg < 2:126513/111‑1 (MQ=255) aaCTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAg > 1:126513/1‑111 (MQ=255) cTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTc > 1:18456/1‑139 (MQ=255) gCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgag < 2:616532/78‑1 (MQ=255) gCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgag > 1:616532/1‑78 (MQ=255) aTTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCag < 2:914934/139‑1 (MQ=255) cGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGt < 1:318725/139‑1 (MQ=255) aCCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATg > 2:1077546/1‑139 (MQ=255) aCCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATg > 1:567767/1‑139 (MQ=255) cAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGa < 2:915249/40‑1 (MQ=255) cAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGa > 1:915249/1‑40 (MQ=255) cAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGga > 2:956912/1‑139 (MQ=255) aaGCGACCCAGAGAACCAATCGCCCATGATTTAgg < 2:908325/35‑1 (MQ=255) aaGCGACCCAGAGAACCAATCGCCCATGATTTAgg > 1:908325/1‑35 (MQ=255) aaGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGgaag > 2:148509/1‑139 (MQ=255) aaGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGgaag > 1:875023/1‑139 (MQ=255) aGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGgaaga > 1:441556/1‑139 (MQ=255) | AACCAGCCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAGA > NC_000913/3901631‑3901899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |