Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,975,924 T→C L260P (CTG→CCG)  wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,975,9240TC100.0% 150.1 / NA 42L260P (CTG→CCG) wecETDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)

CTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCG  >  NC_000913/3975793‑3976058
                                                                                                                                   |                                                                                                                                      
cttcCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCaa                                                                                                                                 <  1:490652/139‑1 (MQ=255)
             gTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATg                                                                                                                    >  1:318819/1‑139 (MQ=255)
                  tAAATATACCTCGCGCGATATTGGCTCCAGCTATTTGATGTCCGCTCTGCAAGCTTCATACCTGTGGGCGCGACTGGACGCATCGGATCGTAACAAACAGCAACGTCTGGCGCCGTGGCAAAACTACTAAGATGCGTTa                                                                                                               >  2:269076/1‑139 (MQ=255)
                        taCCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCct                                                                                                         <  2:809738/139‑1 (MQ=255)
                             ggCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGc                                                                                                    >  1:1041142/1‑139 (MQ=255)
                               cgcgATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGa                                                                                                  <  1:747467/139‑1 (MQ=255)
                               cgcgATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGa                                                                                                  >  2:252537/1‑139 (MQ=255)
                               cgcgATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGa                                                                                                  >  1:876772/1‑139 (MQ=255)
                                   atactGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGc                                                                                              >  1:766632/5‑139 (MQ=255)
                                                         gTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCg                                                                        >  2:902140/1‑139 (MQ=255)
                                                         gTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCg                                                                        >  2:550323/1‑139 (MQ=255)
                                                          tCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAActacta                                                                                                                         <  2:98541/89‑1 (MQ=255)
                                                          tCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAActacta                                                                                                                         >  1:98541/1‑89 (MQ=255)
                                                          tCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGa                                                                       <  2:949566/139‑1 (MQ=255)
                                                              aTCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCGACGTCTGGCGCCGTGGc                                                                                                                                   <  2:946915/75‑1 (MQ=255)
                                                              aTCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGc                                                                                                                                   >  1:946915/1‑75 (MQ=255)
                                                                 tGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGa                                                                <  2:1041142/139‑1 (MQ=255)
                                                                    aaGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATgg                                                             >  1:569219/1‑139 (MQ=255)
                                                                      gCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCt                                                           <  1:902140/139‑1 (MQ=255)
                                                                                  tgGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAAcg                                               >  1:980591/1‑139 (MQ=255)
                                                                                  tgGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAAcg                                               >  2:999373/1‑139 (MQ=255)
                                                                                    ggCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAAcgcg                                             >  2:188286/1‑139 (MQ=255)
                                                                                    ggCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAAcgcg                                             >  1:815791/1‑139 (MQ=255)
                                                                                     gcgcAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACgcgc                                            <  2:876772/139‑1 (MQ=255)
                                                                                      cgcAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACg                                                                                                                       >  2:218453/1‑63 (MQ=255)
                                                                                      cgcAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACg                                                                                                                       <  1:218453/63‑1 (MQ=255)
                                                                                      cgcAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGa                                                  <  1:722440/132‑1 (MQ=255)
                                                                                      cgcAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGa                                                  >  2:722440/1‑132 (MQ=255)
                                                                                       gcAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCat                                          <  2:980591/139‑1 (MQ=255)
                                                                                        cAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCata                                         <  2:318819/139‑1 (MQ=255)
                                                                                          aCTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATg                                       <  1:891661/139‑1 (MQ=255)
                                                                                          aCTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATg                                       <  2:569219/139‑1 (MQ=255)
                                                                                            tGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGtt                                     <  2:815791/139‑1 (MQ=255)
                                                                                            tGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGtt                                     <  2:479146/139‑1 (MQ=255)
                                                                                            tGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTAGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGtt                                     <  2:766632/139‑1 (MQ=255)
                                                                                                   agcGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACAtt                              >  1:491872/1‑139 (MQ=255)
                                                                                                         tCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTg                        <  1:252537/139‑1 (MQ=255)
                                                                                                                          gTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCg       <  2:491872/139‑1 (MQ=255)
                                                                                                                           tCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGa                                                                                                  >  1:521698/1‑47 (MQ=255)
                                                                                                                           tCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGa                                                                                                  <  2:521698/47‑1 (MQ=255)
                                                                                                                            cTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGa     >  1:309950/1‑139 (MQ=255)
                                                                                                                               gcgcCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAgcg  <  1:269076/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
CTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCG  >  NC_000913/3975793‑3976058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑