Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 630,819 C→T A309V (GCG→GTG)  cstA → carbon starvation protein involved in peptide utilization, APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913630,8190CT100.0% 109.2 / NA 32A309V (GCG→GTG) cstAcarbon starvation protein involved in peptide utilization, APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

GCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAC  >  NC_000913/630695‑630939
                                                                                                                            |                                                                                                                        
gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAAttt                                                                                                             >  2:757283/1‑138 (MQ=255)
gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAAttt                                                                                                             <  1:757283/138‑1 (MQ=255)
    tgctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGa                                                                                                        <  2:514034/139‑1 (MQ=255)
              gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCa                                                                                                   <  1:233741/134‑1 (MQ=255)
              gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCa                                                                                                   >  2:233741/1‑134 (MQ=255)
              gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGc                                                                                              <  1:1072479/139‑1 (MQ=255)
                ggTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGccc                                                                                            >  1:1087196/1‑139 (MQ=255)
                      tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATg                                                                                      <  2:66373/139‑1 (MQ=255)
                      tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATg                                                                                      >  1:66373/1‑139 (MQ=255)
                           gCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCg                                                                                 >  2:337045/1‑139 (MQ=255)
                                gcgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAAc                                                                            <  2:1087196/139‑1 (MQ=255)
                                          ctctctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                  <  2:162693/139‑1 (MQ=255)
                                             tctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTcc                                                               >  1:777758/1‑139 (MQ=255)
                                             tctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTcc                                                               <  1:291231/139‑1 (MQ=255)
                                                   ttCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTa                                                         <  1:337045/139‑1 (MQ=255)
                                                    tCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                        >  1:611563/1‑139 (MQ=255)
                                                    tCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                        >  2:287452/1‑139 (MQ=255)
                                                     ccTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATc                                                       >  1:471717/1‑139 (MQ=255)
                                                                ggACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                            >  1:861321/1‑139 (MQ=255)
                                                                     aTCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGgcg                                       <  1:287452/139‑1 (MQ=255)
                                                                           ggTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCa                                                                                                                  >  2:161744/1‑58 (MQ=255)
                                                                           ggTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCa                                                                                                                  <  1:161744/58‑1 (MQ=255)
                                                                                ggCGGTAGGCATTTTGATTATGCGCCCGACGCTGACTATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGAAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                            >  1:714344/1‑139 (MQ=255)
                                                                                 gCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCa                                                                                                   <  2:19304/67‑1 (MQ=255)
                                                                                 gCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCa                                                                                                   >  1:19304/1‑67 (MQ=255)
                                                                                           ttttGATTATGCGCCCGACGCTGACCATGCCTGTGCTGAc                                                                                                                    <  1:473224/40‑1 (MQ=38)
                                                                                           ttttGATTATGCGCCCGACGCTGACCATGCCTGTGCTGAc                                                                                                                    >  2:473224/1‑40 (MQ=38)
                                                                                                aTTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtct            >  1:194879/1‑139 (MQ=255)
                                                                                                aTTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtct            <  2:777758/139‑1 (MQ=255)
                                                                                                      cgcCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCATTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTg      >  2:711121/1‑139 (MQ=255)
                                                                                                          cGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGTCCGGGATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc  <  1:843960/139‑1 (MQ=255)
                                                                                                          cGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc  >  2:935698/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                        
GCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAC  >  NC_000913/630695‑630939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑