| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,533,530 | C→T | L85L (CTG→CTA) | yjhQ ← | GNAT family putative N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,533,530 | 0 | C | T | 95.0% | 121.1 / ‑2.0 | 40 | L85L (CTG→CTA) | yjhQ | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (16/22); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
CGAACCCATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCT > NC_000913/4533394‑4533663 | cGAACGCATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCAGCCCTTGGCATTCCAGTATGACCGCTAg < 2:409422/139‑1 (MQ=255) aaCCCATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAggg < 2:904579/139‑1 (MQ=255) cATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGc < 2:26804/139‑1 (MQ=255) aTGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCg < 2:979189/139‑1 (MQ=255) tAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCa < 1:913546/139‑1 (MQ=255) tggcgtggcCGAGAAC‑AAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGt < 1:100248/139‑1 (MQ=255) tggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAAt > 1:899266/1‑139 (MQ=255) agaACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCAttt < 2:194056/139‑1 (MQ=255) gCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGcc < 1:513272/139‑1 (MQ=255) gCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCCTATCAACCCTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGcc < 2:137880/139‑1 (MQ=255) gCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg < 2:634058/139‑1 (MQ=255) gCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg < 1:890423/139‑1 (MQ=255) aTCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCAGGTATGACCGCTAGGGGAGCGAgg > 2:566555/1‑90 (MQ=255) aTCAACCTCAGGTGCTCGAGGCCAGTGCGTATCAACCGGCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAgg < 1:566555/90‑1 (MQ=255) tCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGa > 1:128821/1‑139 (MQ=255) tCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGa > 2:38087/1‑139 (MQ=255) tATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCa > 1:291294/1‑139 (MQ=255) cAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATAc < 2:127704/139‑1 (MQ=255) cAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATAc < 1:8084/139‑1 (MQ=255) ccGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCgg > 1:773588/1‑139 (MQ=255) gTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGc > 1:648009/1‑139 (MQ=255) tcctccCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGaa > 2:1045762/1‑70 (MQ=255) tcctccCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGaa < 1:1045762/70‑1 (MQ=255) ccACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg < 2:959151/92‑1 (MQ=255) ccACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg > 1:959151/1‑92 (MQ=255) aTCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATAc > 1:1094882/1‑120 (MQ=255) aTCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATAc < 2:1094882/120‑1 (MQ=255) tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTg < 1:3425/67‑1 (MQ=255) tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTg > 2:3425/1‑67 (MQ=255) tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCtt > 2:360647/1‑97 (MQ=255) tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCtt < 1:360647/97‑1 (MQ=255) tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgcgcgc > 1:525625/1‑139 (MQ=255) tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgcgcgc > 1:1115297/1‑139 (MQ=255) cGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCtt > 1:484656/1‑139 (MQ=255) ggTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCttt < 1:884512/139‑1 (MQ=255) tATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCa < 2:773588/139‑1 (MQ=255) tATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCa < 1:38087/139‑1 (MQ=255) tGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc < 2:1115297/139‑1 (MQ=255) tGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc > 2:111282/1‑139 (MQ=255) aCCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCt > 1:909725/1‑139 (MQ=255) | CGAACCCATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCT > NC_000913/4533394‑4533663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |