Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,489	T→C	F59L (TTT→CTT)	nadR →	trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,489	0	T	C	100.0%	255.0 / NA	66	F59L (TTT→CTT)	nadR	trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/39);  total (27/39)

ACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTT  >  NC_000913/4627353‑4627613                                                                                                                                         |                                                                                                                             aCAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGctt                                                                                                                            >  2:109249/1‑139 (MQ=255)    gggCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTccc                                                                                                                         >  2:556949/1‑139 (MQ=255)      gCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCg                                                                                                                       >  2:5485/1‑139 (MQ=255)       cTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCgg                                                                                                                      <  1:556949/139‑1 (MQ=255)            cGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCagaa                                                                                                                 >  1:851447/1‑139 (MQ=255)               tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCATTTTTTGGGGCTTGAGCTTCCCCGGCAgaagaa                                                                                                              >  1:1085175/1‑139 (MQ=255)                   gcagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACg                                                                                                          <  2:1117833/139‑1 (MQ=255)                    gagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGGCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGa                                                                                                         >  1:691179/2‑139 (MQ=255)                     agGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGAt                                                                                                        <  2:889574/139‑1 (MQ=255)                       gTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCg                                                                                                      <  1:449553/139‑1 (MQ=255)                       gTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCg                                                                                                      >  1:291429/1‑139 (MQ=255)                       gTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCg                                                                                                      <  1:648260/139‑1 (MQ=255)                         aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGgt                                                                                                    >  1:406445/1‑139 (MQ=255)                                gCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTAt                                                                                             <  2:851447/139‑1 (MQ=255)                                gCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTAt                                                                                             >  1:39110/1‑139 (MQ=255)                                   aGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCg                                                                                          >  2:864893/1‑139 (MQ=255)                                     cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  2:39110/139‑1 (MQ=255)                                     cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        >  2:991385/1‑139 (MQ=255)                                     cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  1:690611/139‑1 (MQ=255)                                     cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  2:207165/139‑1 (MQ=255)                                     cGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  2:429735/139‑1 (MQ=255)                                            aCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCt                                                                                 <  2:1033532/139‑1 (MQ=255)                                                aaGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGGCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAccc                                                                             >  1:684123/1‑139 (MQ=255)                                                 aGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCagaaga                                                                                                               >  2:499986/1‑104 (MQ=255)                                                 aGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCagaaga                                                                                                               <  1:499986/104‑1 (MQ=255)                                                 aGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        >  1:578838/1‑127 (MQ=255)                                                 aGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  2:578838/127‑1 (MQ=255)                                                    gTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTg                                                                         <  1:109249/139‑1 (MQ=255)                                                       tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCAt                                                                      >  2:176267/1‑139 (MQ=255)                                                         tAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATAc                                                                    <  2:406445/139‑1 (MQ=255)                                                            gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAc                                                                               >  2:745378/1‑125 (MQ=255)                                                            gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAc                                                                               <  1:745378/125‑1 (MQ=255)                                                            gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCgg                                                                 <  1:429769/139‑1 (MQ=255)                                                             ccAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCATTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGa                                                                <  2:1085175/139‑1 (MQ=255)                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGAt                                                                                                        >  2:488688/1‑95 (MQ=255)                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGAt                                                                                                        <  1:488688/95‑1 (MQ=255)                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  2:868431/111‑1 (MQ=255)                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  1:778677/111‑1 (MQ=255)                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  2:795814/111‑1 (MQ=255)                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        >  2:778677/1‑111 (MQ=255)                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        >  1:795814/1‑111 (MQ=255)                                                                 ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        >  1:868431/1‑111 (MQ=255)                                                                  tACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACatat                                                           <  1:434474/139‑1 (MQ=255)                                                                  tACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACatat                                                           <  1:1122080/139‑1 (MQ=255)                                                                           ccAAAATCAAAAGTCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        <  2:749429/101‑1 (MQ=255)                                                                           ccAAAATCAAAAGCCCCAGTGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                        >  1:749429/1‑101 (MQ=255)                                                                                aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTA‑TCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcg                                            <  1:1095537/139‑1 (MQ=255)                                                                                 tCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcg                                            >  2:460118/1‑139 (MQ=255)                                                                                   aaaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACt                                                                          >  1:904859/1‑107 (MQ=255)                                                                                   aaaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACt                                                                          <  2:904859/107‑1 (MQ=255)                                                                                    aaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTc                                                                                                                           <  2:526881/57‑1 (MQ=255)                                                                                    aaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTc                                                                                                                           >  1:526881/1‑57 (MQ=255)                                                                                     aaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATAc                                                                    <  1:473171/111‑1 (MQ=255)                                                                                     aaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATAc                                                                    >  2:473171/1‑111 (MQ=255)                                                                                             gcgcgcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGa                                                                <  2:599666/107‑1 (MQ=255)                                                                                             gcgcgcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGa                                                                >  1:599666/1‑107 (MQ=255)                                                                                                  cAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTg                           <  1:533977/139‑1 (MQ=255)                                                                                                    aaaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGAc                         <  2:684123/139‑1 (MQ=255)                                                                                                     aaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACg                        <  1:991385/139‑1 (MQ=255)                                                                                                       gCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                      <  1:5485/139‑1 (MQ=255)                                                                                                       gCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                      <  2:132717/139‑1 (MQ=255)                                                                                                              gCGTTGCACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCata               <  2:637580/139‑1 (MQ=255)                                                                                                                    cACCGTTTTTTGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTa         <  2:691179/139‑1 (MQ=255)                                                                                                                           ttttGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGttt  >  2:341016/1‑139 (MQ=255)                                                                                                                           ttttGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGttt  <  2:182138/139‑1 (MQ=255)                                                                                                                              tGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                      >  1:572393/1‑116 (MQ=255)                                                                                                                              tGGGGCTTGAGCTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                      <  2:572393/116‑1 (MQ=255)                                                                                                                                         |                                                                                                                             ACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTT  >  NC_000913/4627353‑4627613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑