Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,240,435 | T→C | Q546R (CAG→CGG) | cvrA ← | putative cation/proton antiporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,240,435 | 0 | T | C | 98.2% | 211.1 / NA | 56 | Q546R (CAG→CGG) | cvrA | putative cation/proton antiporter |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (26/29); minor base A (1/0); total (27/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.82e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTG > NC_000913/1240300‑1240557 | aaGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGt < 1:141054/139‑1 (MQ=255) aaGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGt < 1:351704/139‑1 (MQ=255) cGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGa > 1:883789/1‑139 (MQ=255) cGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTg < 2:878720/139‑1 (MQ=255) cGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTg < 2:386151/139‑1 (MQ=255) tAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg < 2:1125005/139‑1 (MQ=255) ttCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCTGCGCCTaa > 2:682866/1‑139 (MQ=255) aaaTTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAActg > 1:276219/1‑139 (MQ=255) aTTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAActgct > 1:874544/1‑139 (MQ=255) tAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGa < 2:874544/139‑1 (MQ=255) aaGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGAc > 2:1027793/1‑139 (MQ=255) aaGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGAc > 1:865376/1‑139 (MQ=255) aGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg > 1:1100451/1‑113 (MQ=255) aGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg < 2:1100451/113‑1 (MQ=255) tctTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg < 1:510637/106‑1 (MQ=255) tctTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg > 2:510637/1‑106 (MQ=255) ttCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGtt < 2:865376/139‑1 (MQ=255) aCCGGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCAGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTgctgct > 2:722411/1‑139 (MQ=255) aCCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTg > 2:391289/1‑94 (MQ=255) aCCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTg < 1:391289/94‑1 (MQ=255) aCCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcgc < 1:507879/98‑1 (MQ=255) aCCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcgc > 2:507879/1‑98 (MQ=255) cGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACg < 1:236824/139‑1 (MQ=255) aTCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATAtt > 2:854956/1‑139 (MQ=255) tCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCg > 2:388774/1‑139 (MQ=255) acacTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGccc < 2:883789/139‑1 (MQ=255) tCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCtt > 1:133359/1‑139 (MQ=255) cATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTc < 2:15159/139‑1 (MQ=255) ttATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa < 1:400821/139‑1 (MQ=255) aTCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg < 2:482662/139‑1 (MQ=255) aTCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg < 2:477881/139‑1 (MQ=255) tcttctTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGc < 1:854956/139‑1 (MQ=255) ttctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCa < 1:509893/139‑1 (MQ=255) tctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCg > 1:97391/1‑126 (MQ=255) tctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCg < 2:97391/126‑1 (MQ=255) tctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCat > 2:264401/1‑139 (MQ=255) tttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatat > 1:474308/1‑139 (MQ=255) tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa > 1:750702/1‑129 (MQ=255) tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa < 2:750702/129‑1 (MQ=255) tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat > 2:770128/1‑139 (MQ=255) gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGc < 2:323717/87‑1 (MQ=255) gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGc > 1:323717/1‑87 (MQ=255) gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcg < 1:1027793/139‑1 (MQ=255) gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcg > 1:844536/1‑139 (MQ=255) ccACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcgc < 1:219830/139‑1 (MQ=255) tCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATc < 1:264401/139‑1 (MQ=255) atcCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGc > 1:412157/1‑72 (MQ=255) atcCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGc < 2:412157/72‑1 (MQ=255) cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat > 1:173607/1‑117 (MQ=255) cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat < 2:173607/117‑1 (MQ=255) cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatat > 1:932294/1‑115 (MQ=255) cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatat < 2:932294/115‑1 (MQ=255) cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCGGCATATTTg > 2:771295/1‑139 (MQ=255) cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg > 2:79770/1‑139 (MQ=255) cAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATc < 1:309989/113‑1 (MQ=255) cAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATc > 2:309989/1‑113 (MQ=255) | AAGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTG > NC_000913/1240300‑1240557 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |