Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,240,435 T→C Q546R (CAG→CGG)  cvrA ← putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,240,4350TC98.2% 211.1 / NA 56Q546R (CAG→CGG) cvrAputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (26/29);  minor base A (1/0);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTG  >  NC_000913/1240300‑1240557
                                                                                                                                       |                                                                                                                          
aaGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGt                                                                                                                         <  1:141054/139‑1 (MQ=255)
aaGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGt                                                                                                                         <  1:351704/139‑1 (MQ=255)
      cGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGa                                                                                                                   >  1:883789/1‑139 (MQ=255)
               cGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTg                                                                                                          <  2:878720/139‑1 (MQ=255)
               cGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTg                                                                                                          <  2:386151/139‑1 (MQ=255)
                  tAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg                                                                                                       <  2:1125005/139‑1 (MQ=255)
                         ttCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCTGCGCCTaa                                                                                                >  2:682866/1‑139 (MQ=255)
                               aaaTTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAActg                                                                                          >  1:276219/1‑139 (MQ=255)
                                 aTTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAActgct                                                                                        >  1:874544/1‑139 (MQ=255)
                                    tAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGa                                                                                     <  2:874544/139‑1 (MQ=255)
                                     aaGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGAc                                                                                    >  2:1027793/1‑139 (MQ=255)
                                     aaGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGAc                                                                                    >  1:865376/1‑139 (MQ=255)
                                            aGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg                                                                                                       >  1:1100451/1‑113 (MQ=255)
                                            aGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg                                                                                                       <  2:1100451/113‑1 (MQ=255)
                                                   tctTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg                                                                                                       <  1:510637/106‑1 (MQ=255)
                                                   tctTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcg                                                                                                       >  2:510637/1‑106 (MQ=255)
                                                     ttCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGtt                                                                    <  2:865376/139‑1 (MQ=255)
                                                            aCCGGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCAGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTgctgct                                                             >  2:722411/1‑139 (MQ=255)
                                                            aCCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTg                                                                                                          >  2:391289/1‑94 (MQ=255)
                                                            aCCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTg                                                                                                          <  1:391289/94‑1 (MQ=255)
                                                            aCCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcgc                                                                                                      <  1:507879/98‑1 (MQ=255)
                                                            aCCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCcgc                                                                                                      >  2:507879/1‑98 (MQ=255)
                                                                   cGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACg                                                      <  1:236824/139‑1 (MQ=255)
                                                                        aTCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATAtt                                                 >  2:854956/1‑139 (MQ=255)
                                                                            tCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCg                                             >  2:388774/1‑139 (MQ=255)
                                                                               acacTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGccc                                          <  2:883789/139‑1 (MQ=255)
                                                                                    tCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCtt                                     >  1:133359/1‑139 (MQ=255)
                                                                                     cATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTc                                    <  2:15159/139‑1 (MQ=255)
                                                                                       ttATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa                                  <  1:400821/139‑1 (MQ=255)
                                                                                         aTCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg                                <  2:482662/139‑1 (MQ=255)
                                                                                         aTCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg                                <  2:477881/139‑1 (MQ=255)
                                                                                          tcttctTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGc                               <  1:854956/139‑1 (MQ=255)
                                                                                            ttctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCa                             <  1:509893/139‑1 (MQ=255)
                                                                                             tctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCg                                         >  1:97391/1‑126 (MQ=255)
                                                                                             tctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCg                                         <  2:97391/126‑1 (MQ=255)
                                                                                             tctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCat                            >  2:264401/1‑139 (MQ=255)
                                                                                               tttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatat                          >  1:474308/1‑139 (MQ=255)
                                                                                                 tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa                                  >  1:750702/1‑129 (MQ=255)
                                                                                                 tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa                                  <  2:750702/129‑1 (MQ=255)
                                                                                                 tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat                        >  2:770128/1‑139 (MQ=255)
                                                                                                      gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGc                                                                       <  2:323717/87‑1 (MQ=255)
                                                                                                      gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGc                                                                       >  1:323717/1‑87 (MQ=255)
                                                                                                      gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcg                   <  1:1027793/139‑1 (MQ=255)
                                                                                                      gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcg                   >  1:844536/1‑139 (MQ=255)
                                                                                                       ccACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcgc                  <  1:219830/139‑1 (MQ=255)
                                                                                                             tCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATc            <  1:264401/139‑1 (MQ=255)
                                                                                                                     atcCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGc                                                                       >  1:412157/1‑72 (MQ=255)
                                                                                                                     atcCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGc                                                                       <  2:412157/72‑1 (MQ=255)
                                                                                                                       cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat                        >  1:173607/1‑117 (MQ=255)
                                                                                                                       cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat                        <  2:173607/117‑1 (MQ=255)
                                                                                                                       cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatat                          >  1:932294/1‑115 (MQ=255)
                                                                                                                       cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatat                          <  2:932294/115‑1 (MQ=255)
                                                                                                                       cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCGGCATATTTg  >  2:771295/1‑139 (MQ=255)
                                                                                                                       cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg  >  2:79770/1‑139 (MQ=255)
                                                                                                                            cAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATc                       <  1:309989/113‑1 (MQ=255)
                                                                                                                            cAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATc                       >  2:309989/1‑113 (MQ=255)
                                                                                                                                       |                                                                                                                          
AAGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTG  >  NC_000913/1240300‑1240557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑