Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,233,485 (T)6→7 coding (608/720 nt) sanA → DUF218 superfamily vancomycin high temperature exclusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,233,4791.T100.0% 90.0 / NA 27R201L (CGT→CTT) sanADUF218 superfamily vancomycin high temperature exclusion protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (14/13);  total (14/13)

TGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑TTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACA  >  NC_000913/2233345‑2233610
                                                                                                                                       |                                                                                                                                   
tGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑tttt                                                                                                                                 >  1:19494/1‑139 (MQ=255)
  cgcTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑tttttt                                                                                                                               >  1:163981/1‑139 (MQ=255)
   gcTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑ttttttt                                                                                                                              >  1:45933/1‑138 (MQ=255)
        atatGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGc                                                                                                                          <  1:419670/139‑1 (MQ=255)
                       cTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTc                                                                                                           <  2:156585/139‑1 (MQ=255)
                        tCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTcc                                                                                                          >  2:109053/1‑139 (MQ=255)
                            tgtTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCt                                                                                                      >  1:104338/1‑139 (MQ=255)
                                aTGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGc                                                                                                  <  2:45933/139‑1 (MQ=255)
                                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAgg                                                                                            >  2:439580/1‑139 (MQ=255)
                                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATA‑TTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTc                                                                                                                  >  1:16604/1‑116 (MQ=255)
                                      tACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATA‑TTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTc                                                                                                                  <  2:16604/116‑1 (MQ=255)
                                               cGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAg                                                                                   <  2:19494/139‑1 (MQ=255)
                                                               aGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATcc                                                                   <  2:294175/139‑1 (MQ=255)
                                                                    gTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCAcc                                                                                               <  1:297540/106‑1 (MQ=255)
                                                                    gTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCAcc                                                                                               >  2:297540/1‑106 (MQ=255)
                                                                           tGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAg                                                                                             <  2:102378/101‑1 (MQ=255)
                                                                           tGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAg                                                                                             >  1:102378/1‑101 (MQ=255)
                                                                               tttGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGaa                                                   >  2:166854/1‑139 (MQ=255)
                                                                                  gccgccCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTGCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAg                                                >  1:302647/1‑139 (MQ=255)
                                                                                                  cgcTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCTGCCGTCACACCCGAACAGTTACTTGAATTACaaa                                <  2:104338/139‑1 (MQ=255)
                                                                                                               ttATATTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGaaa                   <  1:416661/139‑1 (MQ=255)
                                                                                                                 atatTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGaaa                   <  1:450479/137‑1 (MQ=255)
                                                                                                                 atatTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGaaa                   >  2:450479/1‑137 (MQ=255)
                                                                                                                 atatTTTTAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAagt                 >  2:481439/1‑139 (MQ=255)
                                                                                                                    tttttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAagtagt              <  2:163981/139‑1 (MQ=255)
                                                                                                                       ttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTAt           <  2:254465/139‑1 (MQ=255)
                                                                                                                       ttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTAt           >  1:8078/1‑139 (MQ=255)
                                                                                                                       ttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTAt           >  2:461081/1‑139 (MQ=255)
                                                                                                                       ttAAACGTGAACCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAAAAACAAGGAAAGTAGTTAt           >  2:255317/1‑139 (MQ=255)
                                                                                                                                aaCCGCGTTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTAca  >  1:407956/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                   
TGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCG‑TTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACA  >  NC_000913/2233345‑2233610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑