Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 632,923 A→G A185A (GCT→GCC ybdH ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913632,9230AG100.0% 52.8 / NA 16A185A (GCT→GCCybdHputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

GCAAAATGATTGCGTTAACTGTTGATTTTGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATG  >  NC_000913/632788‑633057
                                                                                                                                       |                                                                                                                                      
gCAAAATGATTGCGTTAACTGTTGATTTTGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGcc                                                                                                                                     >  1:56707/1‑139 (MQ=255)
        aTTGCGTTAACTGTTGATTTTGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGcaccac                                                                                                                             <  1:373030/139‑1 (MQ=255)
                             gCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAg                                                                                                        <  1:105913/139‑1 (MQ=255)
                                    gCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCa                                                                                                 >  2:157074/1‑139 (MQ=255)
                                                          tttAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATAtt                                                                           >  1:211555/1‑139 (MQ=255)
                                                               cAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGc                                                                      >  2:197495/1‑139 (MQ=255)
                                                                 aGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCgg                                                                    >  1:233838/1‑139 (MQ=255)
                                                                   aCGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTg                                                                  <  2:367007/139‑1 (MQ=255)
                                                                       cgcgAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCAtt                                                              <  2:10384/139‑1 (MQ=255)
                                                                        gcgAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTg                                                             <  1:152785/139‑1 (MQ=255)
                                                                          gAAGGGCTTTCGCATTATTTATCCCCAGTCCCACGGTTAGCGGCAAGGTTCCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCTCCGATCCCAGTCAGCAGATATTGTTGCGGTGCATTGAg                                                           <  1:44609/139‑1 (MQ=255)
                                                                           aaTGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAgg                                                          <  2:56707/139‑1 (MQ=255)
                                                                                                ccccAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGc                                     <  1:39324/139‑1 (MQ=255)
                                                                                                   cAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATCTTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCAcc                                  >  1:406843/1‑139 (MQ=255)
                                                                                                              ttAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCcagc                                                                                  <  2:352902/80‑1 (MQ=255)
                                                                                                              ttAGCGGCAACGTTTCTGGTTGCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCcagc                                                                                  >  1:352902/1‑80 (MQ=255)
                                                                                                                                   gCGGGGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATg  >  2:369728/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GCAAAATGATTGCGTTAACTGTTGATTTTGCTGATCGCTCAGCGCCTGTTCGCTACTGTTTAACAAGACGTCGCGAATGGCTTGCGCATTATTGATCCCCAGTCGCACGGTTAGCGGCAACGTTTCTGGTTGCGGAGCCAGCACCACCGCTTCATACCATTTCGCCAGCGTGTCACCGATCCCCGCCAGCAGATATTGTTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATG  >  NC_000913/632788‑633057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: