Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	752,248	T→C	Y183C (TAC→TGC)	ybgD ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	752,248	0	T	C	100.0%	71.0 / NA	20	Y183C (TAC→TGC)	ybgD	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/7);  total (13/7)

AAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAAT  >  NC_000913/752148‑752382                                                                                                     |                                                                                                                                       aaGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCg                                                                                                  <  2:346030/139‑1 (MQ=255)    agagTCGATAGATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACt                                                                                               >  1:103771/1‑139 (MQ=255)      agTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGc                                                                                             >  2:214408/1‑139 (MQ=255)                                   tGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGaaaa                                                                >  2:416547/1‑139 (MQ=255)                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGAc                                                                                                <  2:364642/105‑1 (MQ=255)                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGAc                                                                                                >  1:364642/1‑105 (MQ=255)                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAg                                                              >  2:295979/1‑139 (MQ=255)                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAg                                                              >  2:215214/1‑139 (MQ=255)                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAg                                                              >  1:380298/1‑139 (MQ=255)                                            ttttATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCg                                                       >  2:67803/1‑139 (MQ=255)                                             tttATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGt                                                      <  1:295979/139‑1 (MQ=255)                                                        cggcgATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCtgatg                                           <  1:214408/139‑1 (MQ=255)                                                                                        ataATCCAGCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCt           <  1:289402/139‑1 (MQ=255)                                                                                                gCACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGACCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTaa   >  1:196023/1‑139 (MQ=255)                                                                                                 cACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGt                                                      <  1:370267/87‑1 (MQ=255)                                                                                                 cACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGt                                                      >  2:370267/1‑87 (MQ=255)                                                                                                 cACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTc                                                >  2:265437/1‑93 (MQ=255)                                                                                                 cACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTc                                                <  1:265437/93‑1 (MQ=255)                                                                                                 cACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAAt  >  2:130857/1‑139 (MQ=255)                                                                                                 cACGCAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAAt  >  2:483376/1‑139 (MQ=255)                                                                                                     |                                                                                                                                       AAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAAT  >  NC_000913/752148‑752382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑