Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,235,563	A→G	Y209C (TAC→TGC)	fadR →	fatty acid metabolism regulon transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,235,563	0	A	G	100.0%	75.6 / NA	22	Y209C (TAC→TGC)	fadR	fatty acid metabolism regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/10);  total (12/10)

GGATGAAAGGGCTGTATACGCGTATTGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGA  >  NC_000913/1235437‑1235691                                                                                                                               |                                                                                                                                 ggATGAAAGGGCTGTATACGCGTATTGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCg                                                                                                                      <  2:279960/139‑1 (MQ=255)  gATGAAAGGGCTGTATACGCGTATTGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGt                                                                                                                     <  1:328678/139‑1 (MQ=255)      aaaGGGCTGTATACGCGTATTGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCt                                                                                                                 <  1:235952/139‑1 (MQ=255)                      gTATTGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCCCAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTgg                                                                                                 >  2:213683/1‑139 (MQ=255)                         ttGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCa                                                                                                          <  2:72346/127‑1 (MQ=255)                         ttGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCa                                                                                                          >  1:72346/1‑127 (MQ=255)                         ttGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCga                                                                                              >  2:300241/1‑139 (MQ=255)                         ttGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCga                                                                                              >  1:75679/1‑139 (MQ=255)                         ttGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCga                                                                                              >  2:244318/1‑139 (MQ=255)                                                         cgcAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCTgg                                                              >  2:302783/1‑139 (MQ=255)                                                         cgcAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCgg                                                              >  2:411329/1‑139 (MQ=255)                                                         cgcAGTCTGGCGCTGGGCTTCTA‑CACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCggg                                                             >  1:17572/1‑139 (MQ=255)                                                             gTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGa                                                          <  1:244318/139‑1 (MQ=255)                                                                  gcgcTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCTCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAg                                                     <  2:298344/139‑1 (MQ=255)                                                                        ggCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGAt                                                         <  1:193537/129‑1 (MQ=255)                                                                        ggCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGAt                                                         >  2:193537/1‑129 (MQ=255)                                                                        ggCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTc                                               >  2:288821/1‑139 (MQ=255)                                                                        ggCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTc                                               >  1:444629/1‑139 (MQ=255)                                                                                     aaCTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATc                                  >  1:379555/1‑139 (MQ=255)                                                                                                ttGTGCAGTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGAGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTa                       <  1:213683/139‑1 (MQ=255)                                                                                                       gTGAAGGCGCGCACGATCAGGTGTGCGAAACAGTGCGTCGCGATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCa                <  1:300241/139‑1 (MQ=255)                                                                                                                     gATCAGGTGTGCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGa  <  2:98159/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                 GGATGAAAGGGCTGTATACGCGTATTGGTCGTCACTATTTCGCCAATCCGGAAGCGCGCAGTCTGGCGCTGGGCTTCTACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGA  >  NC_000913/1235437‑1235691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑