Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,334,893	A→G	intergenic (+64/‑231)	cysB → / → ymiA	N‑acetylserine‑responsive cysteine regulon transcriptional activator, autorepressor/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,334,893	0	A	G	100.0%	99.3 / NA	28	intergenic (+64/‑231)	cysB/ymiA	N‑acetylserine‑responsive cysteine regulon transcriptional activator, autorepressor/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

TGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTT  >  NC_000913/1334757‑1335006                                                                                                                                         |                                                                                                                  tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                 <  1:256112/139‑1 (MQ=255) tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                 <  2:261515/139‑1 (MQ=255) tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                 <  1:242167/139‑1 (MQ=255) tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                 <  2:43442/139‑1 (MQ=255) tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                 <  2:56801/139‑1 (MQ=255)               tGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGt                                                                                                   <  1:405004/139‑1 (MQ=255)                                gttGAGGTCTATTTGAAAGATATAAGACTGACGGAAAAATAATTTTTTGCGTTATTTTCGGTACTTTTTATGTAGCGTATGTGTCTTAATATATTCTCTTTTTTTGCTTATTTCTATACCGTGCACAATATTTTTATcc                                                                                  <  1:410372/138‑1 (MQ=255)                                aTTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                 >  2:236640/1‑108 (MQ=255)                                aTTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATcc                                                                                  <  1:277835/139‑1 (MQ=255)                                    aGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCa                                                                              <  1:372985/139‑1 (MQ=255)                                     ggTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCat                                                                             >  2:280444/1‑139 (MQ=255)                                          tGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTta                                                                        <  1:390279/139‑1 (MQ=255)                                          tGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTta                                                                        <  2:91055/139‑1 (MQ=255)                                                  gATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTaa                                                                <  1:363696/139‑1 (MQ=255)                                                     ataAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCt                                                                                                                 >  1:45792/1‑87 (MQ=255)                                                             gCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTaa                                                     >  2:235982/1‑139 (MQ=255)                                                              ccGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCt                                                                                >  2:431839/1‑111 (MQ=255)                                                              ccGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCt                                                                                <  1:431839/111‑1 (MQ=255)                                                               cGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATACTTATAGGGTAAATTACTTGTAAag                                                   <  2:305480/139‑1 (MQ=255)                                                                                                     aTGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCa             <  1:483736/139‑1 (MQ=255)                                                                                                          gCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAatat        >  2:115498/1‑139 (MQ=255)                                                                                                          gCGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAatat        >  1:268533/1‑139 (MQ=255)                                                                                                           cGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATc       >  2:28026/1‑139 (MQ=255)                                                                                                           cGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATc       >  1:441352/1‑139 (MQ=255)                                                                                                           cGAAGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATc       >  2:95605/1‑139 (MQ=255)                                                                                                             aaGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCat                                                                             >  2:345318/1‑67 (MQ=255)                                                                                                             aaGGTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCat                                                                             <  1:345318/67‑1 (MQ=255)                                                                                                                gTGCCGGAATATATTCTCTTTTGTTGCTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTAttt  >  2:420510/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                  TGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATATTCTCTTTTGTTACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTT  >  NC_000913/1334757‑1335006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑