Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,657,767 G→A C68Y (TGC→TAC)  ynfD → DUF1161 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,657,7670GA100.0% 151.6 / NA 44C68Y (TGC→TAC) ynfDDUF1161 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/18);  total (26/18)

GTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGA  >  NC_000913/1657641‑1657898
                                                                                                                              |                                                                                                                                   
gtgAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATAc                                                                                                                         <  1:21779/139‑1 (MQ=255)
  gAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGc                                                                                                                       <  2:372392/139‑1 (MQ=255)
       cATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATaaa                                                                                                                  <  1:229088/139‑1 (MQ=255)
                cGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCtat                                                                                                         >  1:147684/1‑139 (MQ=255)
                cGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCtat                                                                                                         >  2:262715/1‑139 (MQ=255)
                          caGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTATGCTAATGATACGCATAAAATTCTCTATACCCGcacca                                                                                               >  1:203107/1‑139 (MQ=255)
                            gcgcATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCaccacc                                                                                             >  1:13606/1‑139 (MQ=255)
                             cgcATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCAccacca                                                                                            >  2:466210/1‑139 (MQ=255)
                               cATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGc                                                                                          >  1:113844/1‑139 (MQ=255)
                                aTTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCg                                                                                         >  1:348930/1‑139 (MQ=255)
                                              cGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCt                                                                           >  1:237485/1‑139 (MQ=255)
                                              cGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCt                                                                           >  1:216633/1‑139 (MQ=255)
                                              cGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCt                                                                           >  1:304480/1‑139 (MQ=255)
                                              cGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCt                                                                           >  2:115347/1‑139 (MQ=255)
                                              cGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCt                                                                           >  2:344894/1‑139 (MQ=255)
                                              cGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCt                                                                           >  2:495030/1‑139 (MQ=255)
                                               gTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTc                                                                          <  1:401028/139‑1 (MQ=255)
                                                   ccGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCcgc                                                                      <  2:436655/139‑1 (MQ=255)
                                                   ccGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCcgc                                                                      <  2:216633/139‑1 (MQ=255)
                                                   ccGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCcgc                                                                      <  1:466210/139‑1 (MQ=255)
                                                   ccGAAAGCAGCTTCACGTTAAGTAGTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCcgc                                                                      <  1:366464/139‑1 (MQ=255)
                                                        agcagcTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGt                                                                 <  2:113844/139‑1 (MQ=255)
                                                                                 ccAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGa                                        >  1:304576/1‑139 (MQ=255)
                                                                                  cAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAAt                                                                                                                <  1:484462/66‑1 (MQ=255)
                                                                                  cAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAAt                                                                                                                >  2:484462/1‑66 (MQ=255)
                                                                                   aaTGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCAGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAAc                                      <  2:304576/139‑1 (MQ=255)
                                                                                     tGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCg                                    >  1:60193/1‑139 (MQ=255)
                                                                                           ggTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAAt                                                                                                                              >  2:110533/1‑43 (MQ=255)
                                                                                           ggTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAAt                                                                                                                              <  1:110533/43‑1 (MQ=255)
                                                                                            gTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCcgc                                                                      >  1:499640/1‑98 (MQ=255)
                                                                                            gTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCcgc                                                                      <  2:499640/98‑1 (MQ=255)
                                                                                               gATCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAAcaca                          >  2:47070/1‑139 (MQ=255)
                                                                                                aTCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATAcc                                                                                                      <  2:21412/62‑1 (MQ=255)
                                                                                                aTCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATAcc                                                                                                      >  1:21412/1‑62 (MQ=255)
                                                                                                 tCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCg                                    >  1:56541/1‑127 (MQ=255)
                                                                                                 tCAGCCTGATTCCCAGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCg                                    <  2:56541/127‑1 (MQ=255)
                                                                                                      cTGATTCCCAGGTCGTCGGCCATTATGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGa                   <  2:203107/139‑1 (MQ=255)
                                                                                                               aGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCcgc                                                                      <  1:52832/79‑1 (MQ=255)
                                                                                                               aGGTCGTCGGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCcgc                                                                      >  2:52832/1‑79 (MQ=255)
                                                                                                                     tcgGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTa                               >  2:275787/1‑112 (MQ=255)
                                                                                                                     tcgGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTa                               <  1:275787/112‑1 (MQ=255)
                                                                                                                     tcgGCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGatat    >  2:63134/1‑139 (MQ=255)
                                                                                                                     tcgGCCAGTACGCTAATGATACGCCTAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGatat    >  2:305218/1‑139 (MQ=255)
                                                                                                                       ggCCATTACGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGa  >  2:290514/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                   
GTGAGCGCATACAGAGCGATATATCACAGCGCATTATCAATAATGGCGTACCCGAAAGCAGCTTCACGTTAAGTATTGTACCCAATGACCAGGTTGATCAGCCTGATTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGA  >  NC_000913/1657641‑1657898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑