Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,719,281	C→T	V108M (GTG→ATG)	anmK ←	anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,719,281	0	C	T	100.0%	58.3 / NA	18	V108M (GTG→ATG)	anmK	anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

GTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCT  >  NC_000913/1719167‑1719412                                                                                                                   |                                                                                                                                    gTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGt                                                                                                             <  1:265645/139‑1 (MQ=255)  tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGtt                                                                                                            >  2:139921/1‑139 (MQ=255)              tccCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACaa                                                                                                >  1:275457/1‑139 (MQ=255)                   aGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                                                                           <  1:28674/139‑1 (MQ=255)                     gCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCg                                                                                         <  1:139921/139‑1 (MQ=255)                           tCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACta                                                                                   <  1:313039/139‑1 (MQ=255)                                                            ccGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTcc                                                         <  1:13408/132‑1 (MQ=255)                                                            ccGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTcc                                                         >  2:13408/1‑132 (MQ=255)                                                               gtgcgtgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAgg                                               >  2:66229/1‑139 (MQ=255)                                                                    tgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACaa                                                                                                <  2:122995/85‑1 (MQ=255)                                                                    tgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACaa                                                                                                >  1:122995/1‑85 (MQ=255)                                                                    tgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTg                                          >  2:345506/1‑139 (MQ=255)                                                                                          ccAATCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGcc                    <  1:143186/139‑1 (MQ=255)                                                                                              tCTGTAAAGTGTGTGGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAg                >  2:182556/1‑139 (MQ=255)                                                                                                         tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGt                                                                                                             >  1:474834/1‑35 (MQ=255)                                                                                                         tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGt                                                                                                             <  2:474834/35‑1 (MQ=255)                                                                                                         tgtgGTGCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCaa     <  1:66229/139‑1 (MQ=255)                                                                                                            ggtgCCATGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCt  >  1:54244/1‑139 (MQ=255)                                                                                                                   |                                                                                                                                    GTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCT  >  NC_000913/1719167‑1719412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑