Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,825,480	T→C	D49G (GAC→GGC)	spy ←	periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,480	0	T	C	100.0%	64.4 / NA	18	D49G (GAC→GGC)	spy	periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/9);  total (9/9)

AATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAA  >  NC_000913/1825353‑1825604                                                                                                                                |                                                                                                                             aaTGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGa                                                                                                                   <  2:29068/139‑1 (MQ=255) aaTGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGa                                                                                                                   <  2:255878/139‑1 (MQ=255)                  cgcgGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTtgtg                                                                                                  >  1:155492/1‑139 (MQ=255)                  cgcgGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTtg                                                                                                    <  1:209728/137‑1 (MQ=255)                  cgcgGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTtg                                                                                                    >  2:209728/1‑137 (MQ=255)                                   ggcggACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTcg                                                                                 >  2:109609/1‑139 (MQ=255)                                                        cGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAg                                                            >  2:190382/1‑139 (MQ=255)                                                                      gATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCa                                              <  1:109609/139‑1 (MQ=255)                                                                      gATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCa                                              <  1:490208/139‑1 (MQ=255)                                                                      gATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCAACATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCa                                              <  1:364332/139‑1 (MQ=255)                                                                           cgcgGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGc                                         >  2:423247/1‑139 (MQ=255)                                                                                          tCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGcc                          >  2:153225/1‑139 (MQ=255)                                                                                                 gTCGGTCAGGTTCAGGTCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGcc                   <  1:153225/139‑1 (MQ=255)                                                                                                             caggtCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGca       >  1:113241/1‑139 (MQ=255)                                                                                                                gtCTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGcaaca    >  2:108878/1‑139 (MQ=255)                                                                                                                  cTTTGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCaacaaa  <  1:491452/139‑1 (MQ=255)                                                                                                                     tGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGc                                                                          <  2:415814/64‑1 (MQ=255)                                                                                                                     tGAACATCATGCCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGc                                                                          >  1:415814/1‑64 (MQ=255)                                                                                                                                |                                                                                                                             AATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGACCGAACTTGCCTTTGTGGTGCATCATCGGCTTCGCGTCAGCCGGTGCTGCGGTAGTGGTGTCTGCGGCATGGGCCAGGTTAGCCGCGCCAAGAGCCAGGGTAGAGGCAACAAA  >  NC_000913/1825353‑1825604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑