Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,897,952	G→A	G341S (GGC→AGC)	sdaA →	L‑serine dehydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,897,952	0	G	A	100.0%	71.0 / NA	22	G341S (GGC→AGC)	sdaA	L‑serine dehydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/8);  total (14/8)

CTATGACCACTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTT  >  NC_000913/1897828‑1898083                                                                                                                             |                                                                                                                                    cTATGACCACTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGtt                                                                                                                       >  1:244424/1‑139 (MQ=255)          cTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCa                                                                                                              >  1:429500/1‑139 (MQ=255)           tttATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCaa                                                                                                             >  1:275109/1‑139 (MQ=255)                     cGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCtgctgc                                                                                                   >  1:136498/1‑139 (MQ=255)                                   aTCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAAc                                                                                     <  2:42823/139‑1 (MQ=255)                                   aTCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAAc                                                                                     <  1:462721/139‑1 (MQ=255)                                              ttACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGt                                                                          >  2:269282/1‑139 (MQ=255)                                                       ggCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTTTTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAg                                                                                                                               <  1:467749/77‑1 (MQ=255)                                                       ggCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTTTTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAg                                                                                                                               >  2:467749/1‑77 (MQ=255)                                                              ggCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGttt                                                          >  2:378594/1‑139 (MQ=255)                                                                                     aaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATg                                   >  1:466970/1‑139 (MQ=255)                                                                                          aCGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCgg                                                                                                 <  2:227432/72‑1 (MQ=255)                                                                                          aCGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCgg                                                                                                 >  1:227432/1‑72 (MQ=255)                                                                                          aCGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCgg                                                                           >  2:176184/1‑94 (MQ=255)                                                                                          aCGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCgg                                                                           <  1:176184/94‑1 (MQ=255)                                                                                          aCGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCAt                                    <  1:408318/133‑1 (MQ=255)                                                                                          aCGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCAt                                    >  2:408318/1‑133 (MQ=255)                                                                                          aCGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAaca                              <  1:277843/139‑1 (MQ=255)                                                                                               tctATTTCCGGTGCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAAcc                         >  2:224930/1‑139 (MQ=255)                                                                                                           gCGGAAGTTGGTTGCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCt             >  2:382519/1‑139 (MQ=255)                                                                                                                 gttggttgCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGAcc       <  1:269282/139‑1 (MQ=255)                                                                                                                      ttgCCAGAGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGtt  >  2:390481/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                    CTATGACCACTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGGAACACAACCTTGGTTTAACCTGCGACCCGGTT  >  NC_000913/1897828‑1898083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑