Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,787	T→C	S34P (TCT→CCT)	mntP →	putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,787	0	T	C	100.0%	130.1 / NA	35	S34P (TCT→CCT)	mntP	putative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/17);  total (18/17)

GATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATA  >  NC_000913/1905652‑1905921                                                                                                                                        |                                                                                                                                       gatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTg                                                                                                                                     <  1:44306/139‑1 (MQ=255)                   cccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGcc                                                                                                                   >  2:179886/1‑139 (MQ=255)                                      tGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGc                                                                                                                    >  2:14316/1‑119 (MQ=255)                                      tGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGc                                                                                                                    <  1:14316/119‑1 (MQ=255)                                           atCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTg                                                                                                                                     <  2:469956/97‑1 (MQ=255)                                           atCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTg                                                                                                                                     >  1:469956/1‑97 (MQ=255)                                           atCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAAccc                                                                                           >  1:5875/1‑139 (MQ=255)                                             cACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTg                                                                                         >  1:34387/1‑139 (MQ=255)                                               cTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAc                                                                                       >  2:165052/1‑139 (MQ=255)                                                    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgccgc                                                                                  >  1:262447/1‑139 (MQ=255)                                                    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgccgc                                                                                  >  2:475364/1‑139 (MQ=255)                                                        ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCGTAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                              <  1:310783/139‑1 (MQ=255)                                                        ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                              <  2:5875/139‑1 (MQ=255)                                                        ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                              <  1:434240/139‑1 (MQ=255)                                                        ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                              <  1:475364/139‑1 (MQ=255)                                                         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATc                                                                             <  1:179886/139‑1 (MQ=255)                                                             cttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                     >  1:473667/1‑127 (MQ=255)                                                             cttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgc                                                                                     <  2:473667/127‑1 (MQ=255)                                                                  gTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGa                                                                    <  1:105741/139‑1 (MQ=255)                                                                      ggTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATgg                                                                >  1:214052/1‑139 (MQ=255)                                                                              gatggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTa                                                        >  2:423090/1‑139 (MQ=255)                                                                                 ggatgCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagcc                                                     >  1:358007/1‑139 (MQ=255)                                                                                      cATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCg                                                >  2:9051/1‑139 (MQ=255)                                                                                      cATTTGCTGCATCAATCGGTAAAGGTGCCAACCTCCATACACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTAGAAACCCTGACGCCACTGATCGGCTGGGGAATGGGCATGTTAACCAGCCg                                                >  1:90672/1‑139 (MQ=255)                                                                                        tttGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGt                                              <  2:67692/139‑1 (MQ=255)                                                                                         ttGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGtt                                             <  2:364673/139‑1 (MQ=255)                                                                                                    aTCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAAt                                  <  2:34387/139‑1 (MQ=255)                                                                                                                gCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                              <  2:340640/83‑1 (MQ=255)                                                                                                                gCCACCCTCCATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                              >  1:340640/1‑83 (MQ=255)                                                                                                                         cATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtg             >  2:288914/1‑139 (MQ=255)                                                                                                                         cATAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtg             <  2:494017/139‑1 (MQ=255)                                                                                                                          aTAAACCGAAATTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtgt            <  1:9051/139‑1 (MQ=255)                                                                                                                                  aaaTTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                              >  2:167339/1‑65 (MQ=255)                                                                                                                                  aaaTTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                              <  1:167339/65‑1 (MQ=255)                                                                                                                                   aaTTTCCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAG‑CAGCCGGTTTGTCCTTGAATGGAACCAATGGATTGCGTTTGGGGCGCTGata  >  2:136201/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       GATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATA  >  NC_000913/1905652‑1905921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑