Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,769	T→C	intergenic (‑410/‑49)	uvrY ← / → yecF	response regulator in two‑component regulatory system with BarA/DUF2594 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,769	0	T	C	100.0%	66.5 / NA	19	intergenic (‑410/‑49)	uvrY/yecF	response regulator in two‑component regulatory system with BarA/DUF2594 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/6);  total (13/6)

ACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAT  >  NC_000913/1995686‑1995903                                                                                    |                                                                                                                                       aCGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTa                                                                                 >  1:431020/1‑139 (MQ=255)    tATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGc                                                                              >  1:59875/1‑139 (MQ=255)                cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTa                                                                                 <  2:467892/124‑1 (MQ=255)                cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTa                                                                                 >  1:467892/1‑124 (MQ=255)                cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  <  2:59875/139‑1 (MQ=255)                cTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  >  2:401594/1‑139 (MQ=255)                   ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  <  2:280962/136‑1 (MQ=255)                   ccGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTa                                                                  >  1:280962/1‑136 (MQ=255)                    cGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGc                                                              <  1:368841/139‑1 (MQ=255)                             gCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGaataa                                                     >  1:298975/1‑139 (MQ=255)                                                                       ataCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCt           <  1:401594/139‑1 (MQ=255)                                                                         aCTCCGCCGCCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAAAAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGa         >  2:215458/1‑139 (MQ=255)                                                                             cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAGTGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCt     >  2:21221/1‑139 (MQ=255)                                                                             cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCt     >  2:384174/1‑139 (MQ=255)                                                                             cgccgcCCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGAAGCt     >  1:311461/1‑139 (MQ=255)                                                                               ccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGtctc                             >  2:189096/1‑113 (MQ=255)                                                                               ccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGtctc                             <  1:189096/113‑1 (MQ=255)                                                                               ccgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGa   >  1:165284/1‑139 (MQ=255)                                                                                cgccCTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAt  >  1:403909/1‑139 (MQ=255)                                                                                    |                                                                                                                                       ACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGAT  >  NC_000913/1995686‑1995903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑