Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,072,728	G→A	G397D (GGT→GAT)	flu →	CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,072,728	0	G	A	100.0%	293.5 / NA	83	G397D (GGT→GAT)	flu	CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/37);  total (46/37)

CTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACT  >  NC_000913/2072592‑2072857                                                                                                                                         |                                                                                                                                  cTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGTGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATa                                                                                                                                 >  2:489016/1‑139 (MQ=255)     ccGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACGGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCg                                                                                                                             >  1:291831/1‑139 (MQ=255)     ccGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCg                                                                                                                             >  2:123813/1‑139 (MQ=255)      cGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCgg                                                                                                                            >  2:14929/1‑139 (MQ=255)      cGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCgg                                                                                                                            >  1:21242/1‑139 (MQ=255)        gACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAg                                                                                                                          <  2:338425/139‑1 (MQ=255)         acacacAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGc                                                                                                                         >  1:266589/1‑139 (MQ=255)           acacaGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCCCCACCACCGCATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGa                                                                                                                       >  1:439804/1‑139 (MQ=255)               aGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGa                                                                                                                   <  1:123813/139‑1 (MQ=255)                gCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCATTGATACCCGGAGCGACGGaa                                                                                                                  <  1:294623/139‑1 (MQ=255)                gCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGaa                                                                                                                  <  2:433571/139‑1 (MQ=255)                gCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGaa                                                                                                                  >  2:350828/1‑139 (MQ=255)                gCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGaa                                                                                                                  >  2:381594/1‑139 (MQ=255)                   aCTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAgg                                                                                                               <  2:147359/139‑1 (MQ=255)                   aCTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAgg                                                                                                               <  2:275101/139‑1 (MQ=255)                     tAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCa                                                                                                             <  2:223254/139‑1 (MQ=255)                       aTACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCAtt                                                                                                           <  2:369701/139‑1 (MQ=255)                           ccGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGt                                                                                                       >  2:374502/1‑139 (MQ=255)                             gcgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTAt                                                                                                     >  2:23971/1‑139 (MQ=255)                              cgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATc                                                                                                    >  2:410865/1‑139 (MQ=255)                               gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCg                                                                                                   >  2:381928/1‑139 (MQ=255)                               gTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCg                                                                                                   >  2:121238/1‑139 (MQ=255)                                        ggCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTc                                                                                          <  2:266589/139‑1 (MQ=255)                                         gCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCa                                                                                         <  2:488588/139‑1 (MQ=255)                                            gAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGc                                                                                      <  1:121238/139‑1 (MQ=255)                                               cGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGa                                                                                   <  1:350828/139‑1 (MQ=255)                                                   ggATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGcc                                                                               <  2:291831/139‑1 (MQ=255)                                                    gATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGccc                                                                              >  1:454192/1‑139 (MQ=255)                                                       gTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGa                                                                           >  2:286316/1‑139 (MQ=255)                                                         ccGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCgg                                                                                    >  2:315008/1‑128 (MQ=255)                                                         ccGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCgg                                                                                    <  1:315008/128‑1 (MQ=255)                                                          cGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGc                                                                        >  2:407826/1‑139 (MQ=255)                                                          cGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGc                                                                        <  1:14929/139‑1 (MQ=255)                                                            cAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTg                                                                      >  2:120947/1‑139 (MQ=255)                                                                     cACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGc                                                             <  2:449394/139‑1 (MQ=255)                                                                         gCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGtt                                                         >  2:85197/1‑139 (MQ=255)                                                                              caccGTATCCATGGGAAATGGCGGTGTACTACTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAg                                                                                                <  1:460714/95‑1 (MQ=255)                                                                              caccGTATCCATGGGAAATGGCGGTGTACTACTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAg                                                                                                >  2:460714/1‑95 (MQ=255)                                                                                 cGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACg                                                 <  1:206451/139‑1 (MQ=255)                                                                                   tATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCt                                               >  1:150077/1‑139 (MQ=255)                                                                                     tCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGa                                             >  2:248760/1‑139 (MQ=255)                                                                                         tGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGc                                         >  2:230689/1‑139 (MQ=255)                                                                                          gggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGcc                                        >  2:303201/1‑139 (MQ=255)                                                                                           ggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCg                                                                                     <  1:144287/93‑1 (MQ=255)                                                                                           ggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCg                                                                                     >  2:144287/1‑93 (MQ=255)                                                                                           ggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCg                                       <  2:433805/139‑1 (MQ=255)                                                                                           ggAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCg                                       >  1:238911/1‑139 (MQ=255)                                                                                             aaaTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGt                                     >  2:481371/1‑139 (MQ=255)                                                                                               aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGc                                                                                                                         <  1:103768/53‑1 (MQ=255)                                                                                               aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGc                                                                                                                         >  2:103768/1‑53 (MQ=255)                                                                                               aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGttcatt                                                     >  2:313387/1‑121 (MQ=255)                                                                                               aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGttcatt                                                     <  1:313387/121‑1 (MQ=255)                                                                                               aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGa                                   >  1:128713/1‑139 (MQ=255)                                                                                               aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGATGGCGGTCAGGCGGATGCCCTGAAGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGa                                   >  1:41724/1‑139 (MQ=255)                                                                                               aTGGCGGTGTACTGCTGGCCGATTCCGGTGCCACTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGAGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGa                                   >  1:302695/1‑139 (MQ=255)                                                                                                 ggCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATa                                 <  1:415880/139‑1 (MQ=255)                                                                                                    ggTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACgg                              <  1:120947/139‑1 (MQ=255)                                                                                                     gtgtACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGc                             <  2:480945/139‑1 (MQ=255)                                                                                                     gtgtACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGc                             <  2:35094/139‑1 (MQ=255)                                                                                                     gtgtACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGc                             <  2:5070/139‑1 (MQ=255)                                                                                                      tgtACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGATACCCAGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGcc                            <  1:39698/139‑1 (MQ=255)                                                                                                           tgctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAGAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGa                       <  2:21242/139‑1 (MQ=255)                                                                                                           tgctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGa                       <  2:497370/139‑1 (MQ=255)                                                                                                             ctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATa                     <  2:454192/139‑1 (MQ=255)                                                                                                             ctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATa                     >  1:247649/1‑139 (MQ=255)                                                                                                             ctgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATa                     >  2:392593/1‑139 (MQ=255)                                                                                                              tgGCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATAc                    <  2:108718/139‑1 (MQ=255)                                                                                                               ggCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATAcc                   <  1:230689/139‑1 (MQ=255)                                                                                                                gCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCa                  >  1:139997/1‑139 (MQ=255)                                                                                                                gCCGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCa                  >  1:137100/1‑139 (MQ=255)                                                                                                                  cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACg                >  2:162890/1‑139 (MQ=255)                                                                                                                  cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACg                >  2:237460/1‑139 (MQ=255)                                                                                                                  cGATTCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACg                >  1:195254/1‑139 (MQ=255)                                                                                                                  cGATTCCGGTGCCACTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACg                >  2:283129/1‑139 (MQ=255)                                                                                                                     ttCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGttcat                                                      <  1:99518/98‑1 (MQ=255)                                                                                                                     ttCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGttcat                                                      >  2:99518/1‑98 (MQ=255)                                                                                                                     ttCCGGTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTa             >  1:43287/1‑139 (MQ=255)                                                                                                                         ggTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATg         <  1:248760/139‑1 (MQ=255)                                                                                                                          gTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACgg                              >  1:62855/1‑117 (MQ=255)                                                                                                                          gTGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACgg                              <  2:62855/117‑1 (MQ=255)                                                                                                                           tGCCGCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGc       <  2:195254/139‑1 (MQ=255)                                                                                                                               gCTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGAc   >  2:180867/1‑139 (MQ=255)                                                                                                                                cTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACt  >  2:270765/1‑139 (MQ=255)                                                                                                                                cTGTCAGTGATACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACt  >  1:383503/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                  CTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACT  >  NC_000913/2072592‑2072857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑