Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,073,225 T→C W563R (TGG→CGG)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,073,2250TC100.0% 377.1 / NA 97W563R (TGG→CGG) fluCP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/51);  total (46/51)

ACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACA  >  NC_000913/2073090‑2073358
                                                                                                                                       |                                                                                                                                     
acgctgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGa                                                                                                                                    <  1:355732/139‑1 (MQ=255)
acgctgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGa                                                                                                                                    <  1:126730/139‑1 (MQ=255)
acgctgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGa                                                                                                                                    <  1:249245/139‑1 (MQ=255)
   ctgacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAata                                                                                                                                 >  2:436924/1‑139 (MQ=255)
     gacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATc                                                                                                                               <  1:396930/139‑1 (MQ=255)
     gacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATc                                                                                                                               <  2:77623/139‑1 (MQ=255)
      acgccgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATcc                                                                                                                              >  1:117788/1‑139 (MQ=255)
           gaACGACAGTACCGTCACCTCGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGAt                                                                                                                         <  2:256380/139‑1 (MQ=255)
                aCAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGc                                                                                                                    >  2:3044/1‑139 (MQ=255)
                  aGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCa                                                                                                                  <  2:358978/139‑1 (MQ=255)
                      ccGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGt                                                                                                              <  1:40618/139‑1 (MQ=255)
                      ccGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGt                                                                                                              >  2:38883/1‑139 (MQ=255)
                      ccGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGt                                                                                                              >  2:40618/1‑139 (MQ=255)
                       cGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTg                                                                                                             <  1:122860/139‑1 (MQ=255)
                       cGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTg                                                                                                             <  2:41724/139‑1 (MQ=255)
                          caccacGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAg                                                                                                          <  1:419547/139‑1 (MQ=255)
                           accacGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTTAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGt                                                                                                         <  1:303201/139‑1 (MQ=255)
                           accacGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGt                                                                                                         >  2:235372/1‑139 (MQ=255)
                           accacGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGt                                                                                                         <  2:266942/139‑1 (MQ=255)
                              acGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAata                                                                                                                                 >  2:17966/1‑112 (MQ=255)
                              acGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAata                                                                                                                                 <  1:17966/112‑1 (MQ=255)
                              acGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCa                                                                                                                  >  1:280086/1‑127 (MQ=255)
                              acGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCa                                                                                                                  <  2:280086/127‑1 (MQ=255)
                              acGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCgg                                                                                                      <  2:135981/139‑1 (MQ=255)
                                  aTGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCggtggt                                                                                                  >  1:349352/1‑139 (MQ=255)
                                   tGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACg                                                                                                                     <  2:122499/119‑1 (MQ=255)
                                   tGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACg                                                                                                                     >  1:122499/1‑119 (MQ=255)
                                    gTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGTTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGtggtgg                                                                                                >  1:70172/1‑139 (MQ=255)
                                    gTCATTGCTAAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGtggtgg                                                                                                >  2:335953/1‑139 (MQ=255)
                                     tCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGa                                                                                               <  2:117788/139‑1 (MQ=255)
                                           cTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCt                                                                                         >  1:434770/1‑139 (MQ=255)
                                                cgcgGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAg                                                                                      <  2:84065/137‑1 (MQ=255)
                                                cgcgGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAg                                                                                      >  1:84065/1‑137 (MQ=255)
                                                cgcgGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGcc                                                                                    >  1:205465/1‑139 (MQ=255)
                                                     tACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACTGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGcc                                                                               >  1:91683/1‑139 (MQ=255)
                                                     tACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGa                                                                                                                                    <  2:324451/86‑1 (MQ=255)
                                                     tACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGa                                                                                                                                    >  1:324451/1‑86 (MQ=255)
                                                      aCAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCg                                                                              <  1:394765/139‑1 (MQ=255)
                                                        aGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGa                                                                            <  1:57058/139‑1 (MQ=255)
                                                          cccTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACa                                                                          >  2:76495/1‑139 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGa                                                                                                                                    >  1:163668/1‑76 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGa                                                                                                                                    <  2:163668/76‑1 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGAt                                                                                                                         <  1:342466/87‑1 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGAt                                                                                                                         >  2:342466/1‑87 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATaa                                                                                                                       <  1:53578/89‑1 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATaa                                                                                                                       >  2:53578/1‑89 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCTGTCGGTGGTGGAt                                                                                              <  1:128467/114‑1 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCTGTCGGTGGTGGAt                                                                                              >  2:128467/1‑114 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGt                                                                                                         >  2:318625/1‑103 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGt                                                                                                         <  1:318625/103‑1 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGAt                                                                                              >  2:146667/1‑114 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGAt                                                                                              <  1:146667/114‑1 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTc                                                                     >  2:233116/1‑139 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTc                                                                     >  2:202332/1‑139 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTc                                                                     >  1:267808/1‑139 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTc                                                                     >  2:142271/1‑139 (MQ=255)
                                                               aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTc                                                                     >  2:149159/1‑139 (MQ=255)
                                                                    gACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTc                                                                >  1:340388/1‑139 (MQ=255)
                                                                    gACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTc                                                                >  1:494967/1‑139 (MQ=255)
                                                                            gcaCTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCAc                                                        <  2:47767/139‑1 (MQ=255)
                                                                            gcaCTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCAc                                                        <  1:436924/139‑1 (MQ=255)
                                                                            gcaCTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCAc                                                        <  1:408144/139‑1 (MQ=255)
                                                                            gcaCTGTGCTGAACGGTCACATTGCCCCAACGAATCTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGAGGACCTCAGCCATGCCGGACAGATTCATTTCACCTcccc                                                        <  1:85423/139‑3 (MQ=255)
                                                                                  tgCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCAcc                                                             >  1:95680/1‑128 (MQ=255)
                                                                                  tgCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCAcc                                                             <  2:95680/128‑1 (MQ=255)
                                                                                   gCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGcaca                                                 <  2:78762/139‑1 (MQ=255)
                                                                                     tGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAgg                                               <  2:340388/139‑1 (MQ=255)
                                                                                         cGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGAGCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAg                                           <  2:56021/139‑1 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCGGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTc                                        >  2:298925/1‑139 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCAc                                                        >  1:302113/1‑123 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGcaca                                                 >  2:88512/1‑130 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGcaca                                                 <  1:88512/130‑1 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGca                                                   >  2:168202/1‑128 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGca                                                   <  1:168202/128‑1 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTc                                        >  2:421381/1‑139 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTc                                        >  2:248806/1‑139 (MQ=255)
                                                                                            tGCCATTGACCCCACGAATCTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCAc                                                        <  2:302113/123‑1 (MQ=255)
                                                                                                    aCCCCACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                <  2:70172/139‑1 (MQ=255)
                                                                                                        cACGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAAcc                            >  1:490720/1‑139 (MQ=255)
                                                                                                          cGAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCt                          <  1:8572/139‑1 (MQ=255)
                                                                                                           gAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                <  2:397423/132‑1 (MQ=255)
                                                                                                           gAATGTCACTCTCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGc                                >  1:397423/1‑132 (MQ=255)
                                                                                                                   ctctCGCCTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGaa                 <  2:376301/139‑1 (MQ=255)
                                                                                                                         ccTCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCt           >  2:406751/1‑139 (MQ=255)
                                                                                                                           tCCGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGa         <  1:248806/139‑1 (MQ=255)
                                                                                                                           gccGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGa         <  1:407826/138‑1 (MQ=255)
                                                                                                                             cGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGc                                                                                <  1:486275/66‑1 (MQ=255)
                                                                                                                             cGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGc                                                                                >  2:486275/1‑66 (MQ=255)
                                                                                                                             cGGTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAAc       >  1:234521/1‑139 (MQ=255)
                                                                                                                              ggTGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACg      <  2:205465/139‑1 (MQ=255)
                                                                                                                                tGCCACTCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCCGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTTAAAAACCTGAACGGa    <  1:335026/139‑1 (MQ=255)
                                                                                                                                tGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGa    <  1:104489/139‑1 (MQ=255)
                                                                                                                                tGCCACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGa    <  2:385072/139‑1 (MQ=255)
                                                                                                                                  ccACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGt                                      >  2:42776/1‑103 (MQ=255)
                                                                                                                                  ccACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGt                                      <  1:42776/103‑1 (MQ=255)
                                                                                                                                  ccACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGa    >  2:285789/1‑137 (MQ=255)
                                                                                                                                  ccACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGa    <  1:285789/137‑1 (MQ=255)
                                                                                                                                  ccACCCGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACa  >  1:219251/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                     
ACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACA  >  NC_000913/2073090‑2073358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑