Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	319,348	G→A	L218L (CTC→CTT)	rclA ←	reactive chlorine stress species (RCS) resistance protein, pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	319,348	0	G	A	100.0%	56.3 / NA	17	L218L (CTC→CTT)	rclA	reactive chlorine stress species (RCS) resistance protein, pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/7);  total (10/7)

AACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTGAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGC  >  NC_000913/319226‑319482                                                                                                                           |                                                                                                                                       aaCGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGc                                                                                                                        >  2:11354/1‑139 (MQ=255)        cGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGAGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATc                                                                                                                 <  1:133965/139‑1 (MQ=255)                        acCGGAAGCTATTAACAGTGCTTCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTcgca                                                                                                 <  1:359985/139‑2 (MQ=255)                                          tGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATcc                                                                               >  2:253544/1‑139 (MQ=255)                                                    gCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTccc                                                                     <  2:424769/139‑1 (MQ=255)                                                                tGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACa                                                         >  1:227227/1‑139 (MQ=255)                                                                          gcacttgcacttGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAgctgc                                               <  1:103392/139‑1 (MQ=255)                                                                            acttgcacttgATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGc                                                       <  2:431723/129‑1 (MQ=255)                                                                            acttgcacttgATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGc                                                       >  1:431723/1‑129 (MQ=255)                                                                               tgcacttgATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGCCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTa                                          >  2:207649/1‑139 (MQ=255)                                                                                 cacttgATTTTCATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTaaa                                        >  1:438515/1‑139 (MQ=255)                                                                                            cATGGTGACTGATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCtt                             >  1:458488/1‑139 (MQ=255)                                                                                                      gATTCGCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCaaaa                   >  2:274126/1‑139 (MQ=255)                                                                                                           gCTCCACATGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGc              <  1:274126/139‑1 (MQ=255)                                                                                                               cacaTGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAAc          >  1:47550/1‑139 (MQ=255)                                                                                                                  aTGGGCATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATa       <  1:265378/139‑1 (MQ=255)                                                                                                                       cATTAAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGc  >  1:251783/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                       AACGAAGCGGTAGCCGGTTGACGACCGGAAGCTATTAACAGTGCATCCACCGCCAGTTGGGCGTGCTCGCTATGCACTTGCACTTGATTTTCATGGTGACTGATTCGCTCCACATGGGCATTGAGGATAATATCGACGCCCTGATCGCGTAAAATCGTCGCGATATTATCAGCAATATCCCGATCTTCCCGAGGCAAAAACAGCGAAGCTGCTTCTAAAATGGTTACCTTGCTGCCAAAATTAGCGAACATAGAGGC  >  NC_000913/319226‑319482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑