Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,193,417 T→C D251G (GAC→GGC)  mrp ← antiporter inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,193,4170TC100.0% 54.5 / NA 17D251G (GAC→GGC) mrpantiporter inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/5);  total (12/5)

ACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGG  >  NC_000913/2193287‑2193529
                                                                                                                                  |                                                                                                                
aCCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCggg                                                                                                          >  1:423963/1‑139 (MQ=255)
                                               tCATGTTCTCGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGt                                                           >  1:160097/1‑139 (MQ=255)
                                               tCATGTTCTCGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGt                                                           >  1:40565/1‑139 (MQ=255)
                                                       tcGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCg                                                                                                            >  2:496059/1‑82 (MQ=255)
                                                       tcGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCg                                                                                                            <  1:496059/82‑1 (MQ=255)
                                                       tcGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGaa                                                   >  2:35047/1‑139 (MQ=255)
                                                       tcGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGaa                                                   >  1:234100/1‑139 (MQ=255)
                                                         gACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATg                                                 >  1:54097/1‑139 (MQ=255)
                                                           cAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAAt                                                  >  1:125469/1‑136 (MQ=255)
                                                           cAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAAt                                                  <  2:125469/136‑1 (MQ=255)
                                                              tACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCAcc                                            >  2:487671/1‑139 (MQ=255)
                                                                         ggAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTggcg                                 <  2:241347/139‑1 (MQ=255)
                                                                                cGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAAt                                                  <  2:148043/115‑1 (MQ=255)
                                                                                cGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAAt                                                  >  1:148043/1‑115 (MQ=255)
                                                                                                        cTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATc                          <  2:224232/115‑1 (MQ=255)
                                                                                                        cTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATc                          >  1:224232/1‑115 (MQ=255)
                                                                                                        cTTTTTTCGCATCGATCAGCGCGATGCCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCgg  >  2:464845/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                
ACCGAAAATCGGTTCATGGTGACCACAGTTACTGCAAATATGCACACTCATGTTCTCGACAATACCGAGCACCGGAACCTCGACTTTCTCGAACATCACAATGCCTTTTTTCGCATCGATCAGCGCGATGTCCTGCGGGGTGGTGACCACGACTGCACCGGTTACTGGAATGTTTTGCGCCAGCGTCAATTGAATGTCACCGGTGCCTGGCGGCATATCAAGCACCAGATAGTCGAGATCCGG  >  NC_000913/2193287‑2193529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: