Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,203,362 A→G I1028M (ATA→ATG yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,203,3620AG100.0% 61.6 / NA 17I1028M (ATA→ATGyehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/9);  total (8/9)

CTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATATGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACC  >  NC_000913/2203252‑2203495
                                                                                                              |                                                                                                                                     
cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           <  1:480557/139‑1 (MQ=255)
cTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACcgc                                                                                                           >  2:480557/1‑139 (MQ=255)
                   cccTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCtt                                                                                        >  1:48849/1‑139 (MQ=255)
                       aCGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCgt                                                                                    <  1:229790/139‑1 (MQ=255)
                                       tGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAggggg                                                                    >  2:248323/1‑139 (MQ=255)
                                          tCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGt                                                                 >  1:456173/1‑139 (MQ=255)
                                              tGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTg                                                             >  2:247931/1‑139 (MQ=255)
                                                gCAGATTTTTATTCTCTGTTTGTTAATCATCCCTTTTCCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCa                                                           <  1:456617/139‑1 (MQ=255)
                                                            tcttTGTTTGGTATTCATGCCTTGACCCGTCTGGTTGCTCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTCTTGACTCAAAGGCTTTCGTGTGGCCGTAGAGGGGGAGTTCTGCAATGCGCAAGATg                                               <  1:386231/139‑1 (MQ=255)
                                                                            atcCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGAcctgcc                               >  1:101654/1‑139 (MQ=255)
                                                                            atcCCTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGAcctgcc                               >  1:236879/1‑139 (MQ=255)
                                                                              cccTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     <  1:223607/99‑1 (MQ=255)
                                                                              cccTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAgggg                                                                     >  2:223607/1‑99 (MQ=255)
                                                                                cTTTACCCGTCTGGTTACCCAGCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCg                           <  2:236879/139‑1 (MQ=255)
                                                                                                     gCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGcc      <  2:456173/139‑1 (MQ=255)
                                                                                                     gCGATTAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGcc      <  2:48849/139‑1 (MQ=255)
                                                                                                         ttAATGTGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCAcc  <  2:62958/139‑1 (MQ=255)
                                                                                                              |                                                                                                                                     
CTCCCGCGTCTTGAAACGGCCCTACGTACCACCCGACGCTGGTCGCTGGCAGATTTTCATTCTCTGTTTGTTAATCATCCCTTTACCCGTCTGGTTACCCAGCGATTAATATGGGGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACC  >  NC_000913/2203252‑2203495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: