Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,298,294	G→A	T142I (ACC→ATC)	napB ←	nitrate reductase, small, cytochrome C550 subunit, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,298,294	0	G	A	100.0%	59.3 / NA	18	T142I (ACC→ATC)	napB	nitrate reductase, small, cytochrome C550 subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

CCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATAC  >  NC_000913/2298162‑2298423                                                                                                                                     |                                                                                                                                  ccGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAgg                                                                                                                             >  1:303468/1‑139 (MQ=255)              gcGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCGGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGAt                                                                                                                <  1:386859/139‑1 (MQ=255)               cGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGAtt                                                                                                               >  1:108506/1‑139 (MQ=255)                 tCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATACTAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTga                                                                                                             >  2:396544/1‑138 (MQ=255)                 tCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTgg                                                                                                             >  2:167646/1‑139 (MQ=255)                   cccAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGgcg                                                                                                           <  2:56275/139‑1 (MQ=255)                                                               cTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAacga                                                               >  1:39323/1‑139 (MQ=255)                                                                        aCCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTa                                                                                                      <  1:207899/91‑1 (MQ=255)                                                                        aCCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTa                                                                                                      >  2:207899/1‑91 (MQ=255)                                                                                 aCGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCcgcg                                             <  2:94560/139‑1 (MQ=255)                                                                                   ggTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGcc                                           <  2:184620/139‑1 (MQ=255)                                                                                             tCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCg                                 <  2:108506/139‑1 (MQ=255)                                                                                                              aTTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATaaa                   >  1:56183/1‑136 (MQ=255)                                                                                                              aTTTCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATaaa                   <  2:56183/136‑1 (MQ=255)                                                                                                                 tCCCGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGt             <  2:95113/139‑1 (MQ=255)                                                                                                                    cGTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAgg          >  2:433796/1‑139 (MQ=255)                                                                                                                     gTAACCTTTTGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGa         <  1:409709/139‑1 (MQ=255)                                                                                                                            tttGATGGGATAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATAc  >  1:196518/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                  CCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAAGGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATAC  >  NC_000913/2298162‑2298423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑