Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,314,992	T→C	L502P (CTG→CCG)	rcsD →	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,314,992	0	T	C	100.0%	84.7 / NA	23	L502P (CTG→CCG)	rcsD	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

AGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGT  >  NC_000913/2314866‑2315097                                                                                                                               |                                                                                                          agGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGt                                                                                               <  2:308547/139‑1 (MQ=255) agGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGt                                                                                               <  2:259425/139‑1 (MQ=255)           gatCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATa                                                                                     <  2:351003/139‑1 (MQ=255)                        aaCATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACa                                                                        >  2:152204/1‑139 (MQ=255)                             tGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCtt                                                                   >  2:387932/1‑139 (MQ=255)                                    gcgcTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg                                                            <  2:202154/139‑1 (MQ=255)                                    gcgcTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg                                                            >  2:103075/1‑139 (MQ=255)                                        tGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAg                                                        <  2:15530/139‑1 (MQ=255)                                               aCCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGaaa                                                 >  2:257049/1‑139 (MQ=255)                                                    cacaGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTga                                            >  1:377604/1‑139 (MQ=255)                                                      caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          <  2:8715/139‑1 (MQ=255)                                                      caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          <  1:103075/139‑1 (MQ=255)                                                      caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          <  2:206453/139‑1 (MQ=255)                                                      caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          <  1:387932/139‑1 (MQ=255)                                                      caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          <  1:311410/139‑1 (MQ=255)                                                      caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          <  1:211793/139‑1 (MQ=255)                                                      caGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          <  1:152204/139‑1 (MQ=255)                                                         tCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACgg                                       >  1:97736/1‑139 (MQ=255)                                                         tCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACgg                                       >  1:251294/1‑139 (MQ=255)                                                                                    aaCGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATtg            >  2:451697/1‑139 (MQ=255)                                                                                     aCGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          >  1:475335/1‑108 (MQ=255)                                                                                     aCGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga                                          <  2:475335/108‑1 (MQ=255)                                                                                              aaaGCAAACAACTGGCGAATCAGGCAGATGTGCCGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTtgt  <  1:257049/139‑1 (MQ=255)                                                                                                                               |                                                                                                          AGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTTAATTGATGAAGTTGT  >  NC_000913/2314866‑2315097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑