Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	445,781	A→G	W296R (TGG→CGG)	yajR ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,781	0	A	G	100.0%	69.4 / NA	19	W296R (TGG→CGG)	yajR	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/9);  total (10/9)

TTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACC  >  NC_000913/445660‑445914                                                                                                                          |                                                                                                                                      ttGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCaa                                                                                                                      >  1:96602/1‑139 (MQ=255)                            aTAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACaa                                                                                           >  1:25787/1‑139 (MQ=255)                                     ggCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACg                                                                                                     >  1:152408/1‑120 (MQ=255)                                     ggCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACg                                                                                                     <  2:152408/120‑1 (MQ=255)                                             ggCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTg                                                                          <  1:207838/139‑1 (MQ=255)                                               cTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTgcg                                                                        >  1:456529/1‑139 (MQ=255)                                                                    caAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCg                                                           >  2:13787/1‑131 (MQ=255)                                                                    caAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCg                                                           <  1:13787/131‑1 (MQ=255)                                                                     aaaGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATg                                                  <  2:25787/139‑1 (MQ=255)                                                                                        accaccAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGaa                                                >  1:390426/1‑122 (MQ=255)                                                                                        accaccAGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGaa                                                <  2:390426/122‑1 (MQ=255)                                                                                           accaGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGAc                                       >  1:476183/1‑128 (MQ=255)                                                                                           accaGTTGCCAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGAc                                       <  2:476183/128‑1 (MQ=255)                                                                                                    cAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCag                                                                                                  <  1:377059/60‑1 (MQ=255)                                                                                                    cAGAACTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCag                                                                                                  >  2:377059/1‑60 (MQ=255)                                                                                                       aaCTGCGTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTg                >  2:493356/1‑139 (MQ=255)                                                                                                             gTTTGCGCGTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCa          >  2:321977/1‑139 (MQ=255)                                                                                                                  cgcgTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAg     <  1:321977/139‑1 (MQ=255)                                                                                                                     gTTCCGCAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGAcc  <  2:96602/139‑1 (MQ=255)                                                                                                                          |                                                                                                                                      TTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACC  >  NC_000913/445660‑445914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑