Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,933,613	T→C	T26A (ACA→GCA)	fucA ←	L‑fuculose‑1‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,933,613	0	T	C	100.0%	50.1 / NA	15	T26A (ACA→GCA)	fucA	L‑fuculose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

GCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCAC  >  NC_000913/2933484‑2933738                                                                                                                                  |                                                                                                                              gCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGtt                                                                                                                      <  1:389760/139‑1 (MQ=255)                          accATCAATAAAGACAATATGCGACTCCGTCAGTTTTGCATATGGAATGCCTGTAGGCGTGATCAGCAGCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAgg                                                                                             <  1:47391/138‑1 (MQ=255)                              tCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAg                                                                                         <  2:432273/139‑1 (MQ=255)                                          atatGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGtt                                                                                                                      >  1:116362/1‑98 (MQ=255)                                          atatGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGtt                                                                                                                      <  2:116362/98‑1 (MQ=255)                                                   tCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTc                                                                                                      <  1:282518/105‑1 (MQ=255)                                                   tCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTc                                                                                                      >  2:282518/1‑105 (MQ=255)                                                                                       aTCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTc                                >  2:56891/1‑139 (MQ=255)                                                                                                  ctccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGA‑TCAAAACAGGGCaa                    <  1:8332/138‑1 (MQ=255)                                                                                                    ccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCaat                   >  1:263001/1‑139 (MQ=255)                                                                                                    ccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCaat                   >  2:328267/1‑139 (MQ=255)                                                                                                        aTAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAAtg               >  2:348397/1‑139 (MQ=255)                                                                                                                  cTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCttt     >  1:10235/1‑139 (MQ=255)                                                                                                                    gACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTca   <  1:58412/139‑1 (MQ=255)                                                                                                                     aCGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTcac  >  1:85116/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                              GCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCAC  >  NC_000913/2933484‑2933738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑