| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,007,295 | T→C | V345A (GTG→GCG) | ygeW → | putative carbamoyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,007,295 | 0 | T | C | 92.9% | 89.1 / ‑1.3 | 28 | V345A (GTG→GCG) | ygeW | putative carbamoyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (13/13); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAG > NC_000913/3007175‑3007429 | cgcAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGa > 2:361089/1‑139 (MQ=255) cTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATc > 1:154840/1‑139 (MQ=255) cTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATc < 2:129257/139‑1 (MQ=255) atgatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGc < 1:129967/99‑1 (MQ=255) atgatgGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGc > 2:129967/1‑99 (MQ=255) gatgGAACTGACCCGTGATGACGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTAGCTACAAAGAAGCCAGc > 2:339425/1‑139 (MQ=255) gAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGa < 2:214453/139‑1 (MQ=255) aCTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAg < 2:154840/139‑1 (MQ=255) aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATcgccgc > 1:394566/1‑139 (MQ=255) gCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCga < 1:361089/139‑1 (MQ=255) ctgcctgcCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGt > 1:450557/1‑139 (MQ=255) gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGct > 1:455010/1‑80 (MQ=255) gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGct < 2:455010/80‑1 (MQ=255) gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa > 2:297162/1‑139 (MQ=255) gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa > 2:411180/1‑139 (MQ=255) gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTaa > 1:46923/1‑139 (MQ=255) gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTG‑AGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGa < 2:381810/101‑1 (MQ=255) gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTG‑AGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGa > 1:381810/1‑101 (MQ=255) cTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCaa < 2:450557/139‑1 (MQ=255) gcggcgTATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGc < 2:277267/139‑1 (MQ=255) tATCCTGTAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCt > 2:71174/1‑139 (MQ=255) tAAAGAAGGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa < 2:283552/139‑1 (MQ=255) aagGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGc < 2:322598/132‑1 (MQ=255) aagGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgct > 2:370147/1‑139 (MQ=255) aagGTGAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCAGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGc > 1:322598/1‑132 (MQ=255) gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaag < 1:375771/139‑1 (MQ=255) gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaag < 1:411180/139‑1 (MQ=255) gAAGCGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAaag < 1:297162/139‑1 (MQ=255) | CGCAACACAAAGACTGGCATTGTACTGAAGAGATGATGGAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAG > NC_000913/3007175‑3007429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |