Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,226,690	A→G	T153A (ACC→GCC)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,226,690	0	A	G	100.0%	149.8 / NA	41	T153A (ACC→GCC)	ygjI	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/18);  total (23/18)

GCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATCCCGAACTTCAACT  >  NC_000913/3226564‑3226819                                                                                                                               |                                                                                                                                  gCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACtt                                                                                                                       >  2:269289/1‑139 (MQ=255)            cTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtg                                                                                                            <  2:411693/139‑1 (MQ=255)               ccTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtgctg                                                                                                         >  1:326813/1‑139 (MQ=255)               ccTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAAGGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtgctg                                                                                                         >  1:485834/1‑139 (MQ=255)                         gAATATATTATGACGCCTGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGc                                                                                               >  2:197014/1‑139 (MQ=255)                             atatTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCtctc                                                                                           <  2:275678/139‑1 (MQ=255)                              tattatGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAAc                                                                                                                   >  2:42963/1‑114 (MQ=255)                              tattatGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAAc                                                                                                                   <  1:42963/114‑1 (MQ=255)                                     aCGGCGGTTGCCACCACCATAATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACAttt                                                                                   >  2:340063/1‑139 (MQ=255)                                     aCGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACAttt                                                                                   >  2:415282/1‑139 (MQ=255)                                        ccGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTAc                                                                                >  2:295740/1‑139 (MQ=255)                                        ccGGTTGCCACCACCATTATCAGTAAGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTAc                                                                                >  2:463414/1‑139 (MQ=255)                                                    accaTTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACgg                                                                    <  2:326813/139‑1 (MQ=255)                                                     ccaTTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGgc                                                                   >  1:437778/1‑139 (MQ=255)                                                        ttATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCt                                                                >  1:438111/1‑139 (MQ=255)                                                               tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGACACTGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTggc                                                         >  1:480176/1‑139 (MQ=255)                                                               tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTggc                                                         >  1:10120/1‑139 (MQ=255)                                                                   gTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAGTGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTTgcggcg                                                     >  1:19322/1‑139 (MQ=255)                                                                     gCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTa                                                   <  1:340063/139‑1 (MQ=255)                                                                     gCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTa                                                   <  2:294539/139‑1 (MQ=255)                                                                      cTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtgc                                                                                                           <  2:148088/82‑1 (MQ=255)                                                                      cTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGAtgc                                                                                                           >  1:148088/1‑82 (MQ=255)                                                                      cTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTAc                                                  >  2:353535/1‑139 (MQ=255)                                                                       tGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACa                                                 >  2:190368/1‑139 (MQ=255)                                                                         ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGcc                                                                                                                                 <  1:134650/57‑1 (MQ=255)                                                                         ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGcc                                                                                                                                 >  2:134650/1‑57 (MQ=255)                                                                         ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGc                                               >  2:267133/1‑139 (MQ=255)                                                                         ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGc                                               <  2:10120/139‑1 (MQ=255)                                                                         ttCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGc                                               <  1:415282/139‑1 (MQ=255)                                                                           cGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGct                                                                                              <  2:167360/90‑1 (MQ=255)                                                                           cGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGct                                                                                              >  1:167360/1‑90 (MQ=255)                                                                            gCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCctg                                            <  1:463414/139‑1 (MQ=255)                                                                              cTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCctgct                                          <  1:190368/139‑1 (MQ=255)                                                                                      ccTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGa                                        <  1:462722/133‑1 (MQ=255)                                                                                      ccTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGa                                        >  2:462722/1‑133 (MQ=255)                                                                                        tGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCa                                <  2:36657/139‑1 (MQ=255)                                                                                                        gggCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGAcg                       >  1:85111/1‑132 (MQ=255)                                                                                                        gggCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGAcg                       <  2:85111/132‑1 (MQ=255)                                                                                                          gCGACAATGTTGGGGTCAATTGCCTACGTCACTTAGACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATcc              <  1:492032/139‑1 (MQ=255)                                                                                                          gCGAAAATGTTGGGGCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATcc              >  2:495176/1‑139 (MQ=255)                                                                                                                      gggCCAATTGCCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATCCCGAACTTCAACt  <  1:349148/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                  GCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCTGACGCCATCACCGTTGACGCGATGATCCCGAACTTCAACT  >  NC_000913/3226564‑3226819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑