Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,591,739 A→T I196N (ATC→AAC)  ugpB ← sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,591,7390AT100.0% 52.9 / NA 16I196N (ATC→AAC) ugpBsn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (7/9);  total (7/9)

CCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCC  >  NC_000913/3591611‑3591851
                                                                                                                                |                                                                                                                
cctcGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTg                                                                                                        >  2:85715/1‑139 (MQ=255)
 ctcGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGTACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGc                                                                                                       <  1:315231/139‑1 (MQ=255)
             gATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCg                                                                                           <  1:393592/139‑1 (MQ=255)
                    ttCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGc                                                                                    <  1:85715/139‑1 (MQ=255)
                           gCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCAt                                                                             >  2:247733/1‑139 (MQ=255)
                            cTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAg                                                                                                             >  1:293384/1‑106 (MQ=255)
                            cTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAg                                                                                                             <  2:293384/106‑1 (MQ=255)
                                                        tCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCgg                                                                        <  1:5971/115‑1 (MQ=255)
                                                        tCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCgg                                                                        >  2:5971/1‑115 (MQ=255)
                                                          cGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAg                                                              <  1:430049/123‑1 (MQ=255)
                                                          cGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAg                                                              >  2:430049/1‑123 (MQ=255)
                                                           gTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCg                                             <  2:151333/139‑1 (MQ=255)
                                                                           tgttTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAg                                                              >  1:206828/1‑106 (MQ=255)
                                                                           tgttTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAg                                                              <  2:206828/106‑1 (MQ=255)
                                                                                           cGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTcc             <  2:115266/139‑1 (MQ=255)
                                                                                                      tCCAGGCGCTAAAGTTTTCCAGTTGGTTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATcc  >  2:113962/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                
CCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCC  >  NC_000913/3591611‑3591851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: