Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,940	A→G	S34G (AGC→GGC)	yhiM →	acid resistance protein, inner membrane

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,940	0	A	G	100.0%	128.1 / NA	34	S34G (AGC→GGC)	yhiM	acid resistance protein, inner membrane
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/19);  total (15/19)

ATATTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTT  >  NC_000913/3634811‑3635054                                                                                                                                  |                                                                                                                   atatTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTAtt                                                                                                           <  1:470724/139‑1 (MQ=255)       gCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTtcgtcg                                                                                                     >  1:349523/1‑139 (MQ=255)       gCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTtcgtcg                                                                                                     >  2:213156/1‑139 (MQ=255)        gaGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTcgc                                                                                                    >  1:312001/2‑139 (MQ=255)             aTACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTc                                                                                               <  2:172289/139‑1 (MQ=255)                ccTGAGGAGCTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCAtg                                                                                            >  2:276076/1‑139 (MQ=255)                    aggagTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCt                                                                                        >  2:482902/1‑139 (MQ=255)                           tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                                                                                 >  1:141531/1‑139 (MQ=255)                           tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                                                                                 >  2:479206/1‑139 (MQ=255)                                 gAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcg                                                                           >  1:203785/1‑139 (MQ=255)                                 gAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcg                                                                           <  2:430786/139‑1 (MQ=255)                                  aaCATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcgg                                                                          >  1:150956/1‑139 (MQ=255)                                  aaCATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcgg                                                                          >  2:173551/1‑139 (MQ=255)                                           atCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                 <  2:217833/139‑1 (MQ=255)                                           atCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                 <  1:479206/139‑1 (MQ=255)                                           atCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                 <  1:401203/139‑1 (MQ=255)                                              gggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTgg                                                              <  2:410054/139‑1 (MQ=255)                                               ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTTGCGGCCATATGCCTGGc                                                             >  2:379377/1‑139 (MQ=255)                                               ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                             >  2:357005/1‑139 (MQ=255)                                                                    cTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATttat                                        <  2:1264/139‑1 (MQ=255)                                                                         aTTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCa                                   <  1:8401/139‑1 (MQ=255)                                                                             tGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTc                               >  2:198803/1‑139 (MQ=255)                                                                                 cTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAg                           >  2:189650/1‑139 (MQ=255)                                                                                         gTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcg                   <  2:141531/139‑1 (MQ=255)                                                                                          tATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcgc                  <  1:482902/139‑1 (MQ=255)                                                                                            tCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcg                   <  2:229773/136‑1 (MQ=255)                                                                                            tCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcg                   >  1:229773/1‑136 (MQ=255)                                                                                                 ttAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTa           <  1:76336/139‑1 (MQ=255)                                                                                                   aGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAAt         <  1:276076/139‑1 (MQ=255)                                                                                                      tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg      <  2:150956/139‑1 (MQ=255)                                                                                                      tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg      <  2:388689/139‑1 (MQ=255)                                                                                                        gCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGtt    <  2:349523/139‑1 (MQ=255)                                                                                                        gCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGtt    <  1:189650/139‑1 (MQ=255)                                                                                                          tttGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGtttt  <  1:213156/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                   ATATTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTT  >  NC_000913/3634811‑3635054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑