Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,655,564	G→A	S113S (AGC→AGT)	yhiD ←	putative Mg(2+) transport ATPase, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,655,564	0	G	A	100.0%	84.5 / NA	25	S113S (AGC→AGT)	yhiD	putative Mg(2+) transport ATPase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/11);  total (14/11)

TACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGAGCAGCAAT  >  NC_000913/3655432‑3655698                                                                                                                                     |                                                                                                                                       tACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCa                                                                                                                                  <  2:490827/139‑1 (MQ=255)    cGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATa                                                                                                                               >  2:73494/1‑139 (MQ=255)     gTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATaa                                                                                                                              <  1:461839/139‑1 (MQ=255)        ttACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCa                                                                                                                           >  2:500066/1‑139 (MQ=255)        ttACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCa                                                                                                                           >  2:14980/1‑139 (MQ=255)              ttCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTa                                                                                                                     >  2:64335/1‑139 (MQ=255)                         cAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTc                                                                                                          <  1:493871/139‑1 (MQ=255)                          aGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCa                                                                                                         >  1:372629/1‑139 (MQ=255)                                                        cGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTa                                                                           <  2:372629/139‑1 (MQ=255)                                                                        ggAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTacg                                                           >  1:96139/1‑139 (MQ=255)                                                                                              ggTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTa                                                                           <  1:195417/101‑1 (MQ=255)                                                                                              ggTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTa                                                                           >  2:195417/1‑101 (MQ=255)                                                                                               gTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGa                                    >  2:132381/1‑139 (MQ=255)                                                                                                  atcaCTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATaa                                 >  1:283261/1‑139 (MQ=255)                                                                                                        gAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGa                           <  2:472393/139‑1 (MQ=255)                                                                                                         aaCCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGAc                          >  1:445307/1‑139 (MQ=255)                                                                                                           ccATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGc                        <  1:132381/139‑1 (MQ=255)                                                                                                              tAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCg                     >  1:82065/1‑139 (MQ=255)                                                                                                              tAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCg                     >  2:494399/1‑139 (MQ=255)                                                                                                                aaTACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTc                   <  2:445307/139‑1 (MQ=255)                                                                                                                        gTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTg           <  1:64335/139‑1 (MQ=255)                                                                                                                         ttCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGa          >  1:437566/1‑139 (MQ=255)                                                                                                                             tACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATGTTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGagcag      >  1:191163/1‑139 (MQ=255)                                                                                                                                aTACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGAGCAGCaa   <  2:375243/139‑1 (MQ=255)                                                                                                                                 tACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGAGCAGCAAt  <  2:437566/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGAGCAGCAAT  >  NC_000913/3655432‑3655698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑